Serum levels of prolactin, luteinizing hormone, and follicle stimulating hormone during pregnancy in the rat.
Serum levels of prolactin, luteinizing hormone, and follicle stimulating hormone during pregnancy in the rat.
复制标题
大鼠妊娠期间催乳素、黄体生成素和卵泡刺激素的血清水平。
作者：
D. Linkie;G. Niswender
每组交配和未交配的雌性大鼠在发情当天的0800、1200、1600和2000时被处死。在交配的动物中，这些时间分别对应于交配后的4、8、12和16小时。采用放射免疫法测定两组患者血清催乳素、黄体生成素、卵泡刺激素的水平。交配大鼠的催乳素水平在性交后的整个16小时内升高(p<；0.05)，而促黄体生成素水平仅在前8小时内升高。卵泡刺激素水平的变化不允许对交配后这种激素的释放做出有意义的结论。其他雌性大鼠交配，怀孕第一天被指定为在阴道冲洗液中发现精子的那一天。在足月(第23天)之前，每天间隔一次处死动物群体，并采集血液进行促性腺激素测定。在整个妊娠期间，血清中促性腺激素的水平通常低于在妊娠期间观察到的水平。
Groups of mated and nonmated female rats were decapitated at 0800, 1200, 1600, and 2000 hours on the day of estrus. In the mated animals, these times corresponded to 4, 8, 12, and 16 hours respectively following coitus. Serum levels of prolactin, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were determined in both groups by specific and sensitive radioimmunoassays. Prolactin levels in mated rats were elevated (p < 0.05) during the entire 16- hour period following coitus, whereas increases in LH were noted for only the first 8 hours. Variation in the levels of FSH did not permit a meaningful conclusion regarding release of this hormone following mating. Additional female rats were mated, and day 1 of pregnancy was designated the day sperm were found in the vaginal lavage. Groups of animals were killed at daily intervals until term (day 23) and blood was collected for gonadotropin assays. Levels of gonadotropins in serum throughout gestation were generally less than those observed during...