Status epilepticus induces time-dependent neuronal and astrocytic expression of interleukin-1 receptor type I in the rat limbic system
Status epilepticus induces time-dependent neuronal and astrocytic expression of interleukin-1 receptor type I in the rat limbic system
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DOI：
10.1016/j.neuroscience.2005.07.063
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Vezzani, A
中科院分区：
文献类型：
--
作者：
Ravizza, T;Vezzani, A
诱发癫痫发作后，神经胶质细胞迅速合成白细胞介素 10。最近的证据表明，内源性 IL-1 β 具有由 I 型白细胞介素 1 受体介导的促惊厥作用。该受体还介导白细胞介素 1 13 对神经元对神经毒性损伤的敏感性的影响。在这项研究中，我们研究了大鼠前脑中白细胞介素 1 受体 I 型的基础和癫痫诱导的表达，以鉴定癫痫活动期间白细胞介素 1 β 的靶向细胞。自我维持的边缘系统癫痫持续状态是通过电刺激大鼠腹侧海马体诱导。在对照脑组织的神经元中几乎检测不到 I 型白介素 1 受体免疫反应性。在癫痫持续状态期间，I型白介素-1受体首先在海马神经元中被诱导，几小时后在位于边缘和边缘外区域的星形胶质细胞中被诱导。海马中的神经元白细胞介素 1 受体 I 型表达比自发性脑电图癫痫发作的持续时间更长，并且在退化神经元中没有观察到。星形胶质细胞的表达在癫痫持续状态诱导后 6 至 18 小时内短暂发生，并且总是在神经元损伤区域中发现。癫痫持续状态期间白细胞介素 1 受体 I 型表达的这些时间依赖性、细胞和区域特异性变化表明，神经元中的白细胞介素 1 受体 I 型介导白细胞介素 1 β 诱导的海马兴奋性快速变化，而星形胶质细胞中的白细胞介素 1 受体 I 型受体可能介导IL-1β 对恶劣条件下神经元存活的影响。 (c) 2005 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Interleukin-10 is rapidly synthesized by glia after the induction of seizures. Recent evidence shows that endogenous IL-1 beta has proconvulsant actions mediated by interleukin-1 receptor type I. This receptor also mediates interleukin-1 13 effects on neuronal susceptibility to neurotoxic insults.In this study, we investigated the basal and seizure-induced expression of interleukin-1 receptor type I in rat forebrain to identify the cells targeted by interleukin-1 beta during epileptic activity.Self-sustained limbic status epilepticus was induced in rats by electrical stimulation of the ventral hippocampus. Interleukin-1 receptor type I immunoreactivity was barely detectable in neurons in control brain tissue. During status epilepticus, interleukin-1 receptor type I was induced in the hippocampal neurons firstly, and several hours later in astrocytes localized in limbic and extralimbic areas. Neuronal interleukin-1 receptor type I expression in the hippocampus outlasted the duration of spontaneous electroencephalographic seizure and was not observed in degenerating neurons. Astrocytic expression occurred transiently, between six and 18 h after the induction of status epilepticus and was invariably found in regions of neuronal damage.These time-dependent, cell- and region-specific changes in interleukin-1 receptor type I expression during status epilepticus suggest that interleukin-1 receptor type I in neurons mediates interieukin-1 beta-induced fast changes in hippocampal excitability while interleukin-1 receptor type I receptors in astrocytes may mediate interleukin-1 beta effects on neuronal survival in hostile conditions. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.