Genomic and antigenic characterization of the newly emerging Chinese duck egg-drop syndrome flavivirus: genomic comparison with Tembusu and Sitiawan viruses
Genomic and antigenic characterization of the newly emerging Chinese duck egg-drop syndrome flavivirus: genomic comparison with Tembusu and Sitiawan viruses
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新出现的中国鸭落蛋综合症黄病毒的基因组和抗原特征：与坦布苏病毒和实兆远病毒的基因组比较
DOI：
10.1099/vir.0.043554-0
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发表时间：
2012-10-01
影响因子：
3.8
通讯作者：
Gao, George F.
中科院分区：
文献类型：
--
作者：
Liu, Peipei;Lu, Hao;Gao, George F.
鸭蛋掉落综合症病毒（DEDSV）是我国新出现的一种引起禽类疾病的致病性黄病毒。这种感染发生在产蛋鸭中，其特点是产蛋量严重下降，死亡率为 5-15%。该病毒被发现与从东南亚蚊子中分离出来的坦布苏病毒 (TMUV) 最密切相关。在这里，我们对 7 个 DEDSV 毒株的全长基因组进行了测序和表征，包括 5'- 和 3'- 非编码区 (NCR)。我们还首次报道了 TMUV 和实兆远病毒 (STWV) 的 ORF 序列，后者是从病鸡中分离出的另一种密切相关的黄病毒。我们分析了 DEDSV 与亚洲病毒 TMUV 和 STWV 以及其他代表性黄病毒的系统发育和抗原关系。我们的结果证实了 DEDSV 和 TMUV/STWV 之间的密切关系，并讨论了它们可能的进化起源。我们还表征了这些病毒的切割位点、潜在的糖基化位点和独特的基序/模块。此外，还鉴定了 5'- 和 3'-NCR 中的保守序列，并研究了预测的末端序列二级结构。 DEDSV 与相关致病性黄病毒的抗原交叉反应性比较发现，其与登革热病毒 (DENV) 具有惊人的密切关系，并提出了 DEDSV 是否可能对人类具有潜在感染威胁的问题。重要的是，DEDSV 可以被针对 DENV 衍生的具有广泛交叉反应性的黄病毒单克隆抗体 2A10G6 有效识别。这些研究的意义是在新出现的 DEDSV 的出现、进化、流行病学、抗原性和致病性的背景下讨论的。
Duck egg-drop syndrome virus (DEDSV) is a newly emerging pathogenic flavivirus causing avian diseases in China. The infection occurs in laying ducks characterized by a severe drop in egg production with a fatality rate of 5-15%. The virus was found to be most closely related to Tembusu virus (TMUV), an isolate from mosquitoes in South-east Asia Here, we have sequenced and characterized the full-length genomes of seven DEDSV strains, including the 5'- and 3'-non-coding regions (NCRs). We also report for the first time the ORF sequences of TMUV and Sitiawan virus (STWV), another closely related flavivirus isolated from diseased chickens. We analysed the phylogenetic and antigenic relationships of DEDSV in relation to the Asian viruses TMUV and STWV, and other representative flaviviruses. Our results confirm the close relationship between DEDSV and TMUV/STWV and we discuss their probable evolutionary origins. We have also characterized the cleavage sites, potential glycosylation sites and unique motifs/modules of these viruses. Additionally, conserved sequences in both 5'- and 3'-NCRs were identified and the predicted secondary structures of the terminal sequences were studied. Antigenic cross-reactivity comparisons of DEDSV with related pathogenic flaviviruses identified a surprisingly close relationship with dengue virus (DENV) and raised the question of whether or not DEDSV may have a potential infectious threat to man. Importantly, DEDSV can be efficiently recognized by a broadly cross-reactive flavivirus mAb, 2A10G6, derived against DENV. The significance of these studies is discussed in the context of the emergence, evolution, epidemiology, antigenicity and pathogenicity of the newly emergent DEDSV.