Effect of beta-carotene on the inhibition of lung metastasis in mice.
Effect of beta-carotene on the inhibition of lung metastasis in mice.
复制标题
β-胡萝卜素对小鼠肺转移的抑制作用。
作者：
C. Pradeep;G. Kuttan,
在C57 BL/6小鼠中研究了β-胡萝卜素对B16 F-10黑色素瘤细胞诱导的肺转移的抑制作用。在肿瘤诱导后同时施用化合物产生肿瘤结节形成的显著减少（71.36%）。与正常动物（0.95微克/毫克蛋白质）相比，对照动物转移肺中增加的肺胶原羟脯氨酸（22.37微克/毫克蛋白质）在β-胡萝卜素治疗的动物中显著减少（4.19微克/毫克蛋白质）。转移对照动物中的大量糖醛酸（355.83微克/100毫克组织）在用β-胡萝卜素处理的动物中显著降低（87.87微克/100毫克组织）。与未处理的对照动物（4.2 mg/100 mg冻干组织）相比，β-胡萝卜素处理的动物（1.58 mg/100 mg冻干组织）的肺己糖胺含量也受到显著抑制。未处理的对照动物中血清唾液酸和血清γ谷氨酰转肽酶活性水平的升高在用β-胡萝卜素处理的动物中显著降低。β胡萝卜素治疗的动物存活长达69天。肺组织的组织学也与上述参数和药物处理动物的寿命相关。我们的研究结果揭示了β-胡萝卜素的抗转移活性，β-胡萝卜素大量存在于绿色植物、蔬菜和水果中。
Effect of beta-carotene on the inhibition of lung metastasis induced by B16F-10 melanoma cells was studied in C57BL/6 mice. Simultaneous administration of the compound after tumor induction produced a significant reduction (71.36%) in tumor nodule formation. Increased lung collagen hydroxyproline (22.37 microg/mg protein) in the metastasized lungs of control animals compared to the normal animals (0.95 microg/mg protein) was significantly reduced (4.19 microg/mg protein) in the beta-carotene treated animals. High amount of uronic acid (355.83 microg/100mg tissue ) in the metastasized control animals was significantly reduced (87.87 microg/100 mg tissue) in the animals treated with beta-carotene. Lung hexosamine content also was inhibited significantly in the beta-carotene treated animals (1.58 mg/100 mg lyophilized tissue) compared to the untreated control animals (4.2 mg/100 mg lyophilized tissue). The elevated levels of serum sialic acid and serum gamma glutamyl transpeptidase activity in the untreated control animals was significantly reduced in the animals treated with beta-carotene. Beta carotene treated animals were survived up to 69 days. Histopathology of the lung tissue also correlated with the above parameters and life span of the drug treated animals. Our results reveal the antimetastatic activity of beta-carotene which are abundantly present in green plants, vegetables and fruits.