Critical temperatures in the cephalopod Sepia officinalis investigated using in vivo 31P NMR spectroscopy
Critical temperatures in the cephalopod Sepia officinalis investigated using in vivo 31P NMR spectroscopy
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使用体内 31P NMR 光谱研究头足类乌贼的临界温度
DOI：
10.1242/jeb.02054
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发表时间：
2006
影响因子：
2.8
通讯作者：
H. Pörtner
中科院分区：
文献类型：
--
作者：
F. Melzner;C. Bock;H. Pörtner
摘要本研究的目的是测试的假设，氧气限制定义的耐热性在欧洲乌贼（乌贼）。使用体内31 P NMR光谱监测外套膜肌肉器官代谢状态和pHi，而通过记录通气和自发运动期间的外套膜腔压力振荡来确定外套膜肌肉性能。在对照条件下（15°C），肌肉磷酸-l-精氨酸（PLA）和无机磷酸盐（Pi）水平的变化可能与频繁发生的高压外套膜收缩（压力振幅（MMPA）>0.2 kPa）呈线性相关。因此，> 2kPa的MMPA主要影响肌肉PLA储备，表明MMPA <2kPa的收缩仅涉及有氧环形外套膜肌肉组织的薄层。平均而言，不超过20%的肌肉PLA消耗在自发运动控制条件下。使动物以平均1摄氏度的速率经受急性热变化。h-1导致显著的Pi积累（相当于PLA分解）和ATP水解自由能（dG/dG）在温度窗口两端的减少，分别从7.0和26.8°C的平均临界温度（Tc）开始。频繁组的高压外套膜收缩不能（在温暖）或只有部分（在寒冷）与净PLA在外套膜肌肉的故障，表明氧限制的日常代谢，而不是运动相关的磷酸酶的使用。我们推测，这主要是不断工作的桡侧地幔肌肉，逐渐缺乏氧气。估计非常低的dG/dG值（-44 kJ mol-1），在这个隔间，沿着与相关的停滞通风压力在温暖，支持这一假设。总之，我们发现了在乌贼乌贼耐热氧限制的证据，所示的常规地幔代谢的厌氧模式的能源生产的逐步过渡。
SUMMARY The present study was designed to test the hypothesis of an oxygen limitation defining thermal tolerance in the European cuttlefish (Sepia officinalis). Mantle muscle organ metabolic status and pHi were monitored using in vivo 31P NMR spectroscopy, while mantle muscle performance was determined by recording mantle cavity pressure oscillations during ventilation and spontaneous exercise. Under control conditions (15°C), changes in muscle phospho-l-arginine (PLA) and inorganic phosphate (Pi) levels could be linearly related to frequently occurring, high-pressure mantle contractions with pressure amplitudes (MMPA) of >0.2 kPa. Accordingly, mainly MMPA of >2 kPa affected muscle PLA reserves, indicating that contractions with MMPA of <2 kPa only involve the thin layers of aerobic circular mantle musculature. On average, no more than 20% of muscle PLA was depleted during spontaneous exercise under control conditions. Subjecting animals to acute thermal change at an average rate of 1 deg. h–1 led to significant Pi accumulation (equivalent to PLA breakdown) and decrements in the free energy of ATP hydrolysis (dG/dζ) at both ends of the temperature window, starting at mean critical temperatures (Tc) of 7.0 and 26.8°C, respectively. Frequent groups of high-pressure mantle contractions could not (in the warm) or only partially (in the cold) be related to net PLA breakdown in mantle muscle, indicating an oxygen limitation of routine metabolism rather than exercise-related phosphagen use. We hypothesize that it is mainly the constantly working radial mantle muscles that become progressively devoid of oxygen. Estimates of very low dG/dζ values (–44 kJ mol–1) in this compartment, along with correlated stagnating ventilation pressures in the warm, support this hypothesis. In conclusion, we found evidence for an oxygen limitation of thermal tolerance in the cuttlefish Sepia officinalis, as indicated by a progressive transition of routine mantle metabolism to an anaerobic mode of energy production.
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