Yeast casein kinase I homologues: an essential gene pair.
Yeast casein kinase I homologues: an essential gene pair.
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DOI：
10.1073/pnas.89.1.28
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发表时间：
1992
影响因子：
11.1
通讯作者：
L. C. Robinson;E. Hubbard;P. Graves;A. DePaoli-Roach;P. Roach;C. Kung;D. Haas;C. Hagedorn
中科院分区：
文献类型：
--
作者：
L. C. Robinson;E. Hubbard;P. Graves;A. DePaoli-Roach;P. Roach;C. Kung;D. Haas;C. Hagedorn
我们报告分离的一个重要的对酿酒酵母基因编码蛋白激酶同源物。这两个基因被独立地分离为剂量依赖性抑制因子。增加剂量的YCK 1抑制了由SNF 1蛋白激酶活性降低引起的缺陷，增加剂量的YCK 2减轻了野生型细胞对盐胁迫的敏感性。这两种基因在两种生长试验中的功能相同，并且任一基因单独丧失功能对生长没有明显影响。然而，这两个基因的功能丧失导致不活动。这两个预测的蛋白质产物共享77%的总体氨基酸同一性，并包含蛋白激酶之间保守的序列元件。获得的兔酪蛋白激酶I的部分序列与两种酵母基因产物具有64%的同一性。此外，在来自过表达YCK 2的细胞的提取物中观察到酪蛋白激酶I活性的增加。因此，YCK 1和YCK 2似乎编码酪蛋白激酶I同源物。
We report the isolation of an essential pair of Saccharomyces cerevisiae genes that encode protein kinase homologues. The two genes were independently isolated as dosage-dependent suppressors. Increased dosage of YCK1 suppressed defects caused by reduced SNF1 protein kinase activity, and increased dosage of YCK2 relieved sensitivity of wild-type cells to salt stress. The two genes function identically in the two growth assays, and loss of function of either gene alone has no discernible effect on growth. However, loss of function of both genes results in inviability. The two predicted protein products share 77% overall amino acid identity and contain sequence elements conserved among protein kinases. Partial sequence obtained for rabbit casein kinase I shares 64% identity with the two yeast gene products. Moreover, an increase in casein kinase I activity is observed in extracts from cells overexpressing YCK2. Thus YCK1 and YCK2 appear to encode casein kinase I homologues.