Electrophysiological responses of galeal contact chemoreceptors of Apis mellifera to selected sugars and electroylytes.
Electrophysiological responses of galeal contact chemoreceptors of Apis mellifera to selected sugars and electroylytes.
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DOI：
10.1016/0022-1910(76)90052-4
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发表时间：
1976
影响因子：
2.2
通讯作者：
Armand T. Whitehead;Joseph R. Larsen
中科院分区：
文献类型：
--
作者：
Armand T. Whitehead;Joseph R. Larsen
用常规的电生理方法，蜜蜂的盖氏感受器对蔗糖、葡萄糖、果糖、氯化钠、氯化钾和氯化锂的溶质浓度呈线性反应，而对氯化钙和氯化镁的反应不一致。这些感受器对糖的激发速率比盐溶液高得多；它们对较低浓度的反应是：氯化钠；氯化钾；氯化锂；⪡果糖；葡萄糖；⪡蔗糖。在较高浓度下，氯化钠、氯化锂、氯化钾、⪡葡萄糖、果糖、⪡蔗糖。观察到四种不同的尖峰类型。第一种类型的波幅最大，是糖刺激的结果。第二种类型的身高较低，发生在盐刺激的前30秒。经KCl长时间刺激后，出现了高度最低的第三种类型和第二种类型。第四类是机械刺激所致的高波幅。感受器对糖溶液的适应与时间的对数呈线性关系，与盐浓度的对数呈非线性关系。葡萄糖-果糖混合糖溶液对反应有协同作用，而蔗糖溶液与葡萄糖和/或果糖混合时则有抑制作用。感受器对机械刺激的反应具有时相-紧张性特征。被测试的感受器对水都没有反应。
Using conventional electrophysiological methods, the galeal sensilla chaetica of the honey bee,Apis mellifera, responded linearly to the log of solute concentrations of sucrose, glucose, fructose, NaCl, KCl, and LiCl but not to CaCl2or MgCl2, which failed to give consistent responses. These sensillae had much higher firing rates for sugar than salt solutions; their relative responses to lower concentrations being NaCl < KCl < LiCl ⪡ fructose < glucose ⪡ sucrose. At higher concentrations NaCl < LiCl < KCl ⪡ glucose < fructose ⪡ sucrose. Four different spike types were seen. The first type had the highest amplitude and resulted from sugar stimulation. The second type had a lower height and occurred in the first 30 sec of salt stimulation. A third type with the lowest height appeared with those of the second type after prolonged stimulation with KCl. A fourth type with a high amplitude resulted from mechanical stimulation. The sensilla adapted to sugar solutions linearly to the logarithm of time and non-linearly to the log of salt concentrations. Glucose-fructose mixed-sugar solution effected synergism of response while sucrose solutions caused inhibition when mixed with glucose and/or fructose. Responses of the sensilla to mechanical stimulation showed phasic-tonic characteristics. None of the sensilla tested responded to water.