MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia
MiR-21 involve in ERK-mediated upregulation of MMP9 in the rat hippocampus following cerebral ischemia
复制标题
MiR-21参与脑缺血后大鼠海马中ERK介导的MMP9上调
DOI：
10.1016/j.brainresbull.2013.02.007
复制
发表时间：
2013-05-01
影响因子：
3.8
通讯作者：
Guo, Jun
中科院分区：
文献类型：
--
作者：
Deng, XiaHeng;Zhong, Yun;Guo, Jun
Matrix metallinoprotease-9 (MMP9) plays a key role in the pathogenesis of post-ischemic blood brain barrier (BBB) disruption and the formation of lesions after cerebral ischemia. In this study we investigate the effect of brain-specific miRNAs on MMP-9 protein level in the rat hippocampus following cerebral ischemia and its underlying mechanism. Cerebral ischemia significantly upregulated miR-21 and -224 in the hippocampus; however, expression of miR-122 and -338-3p was not significantly affected by ischemia. Silencing of miR-21, but not -224, reduced MMP9 protein level after cerebral ischemia. Downregulation of extracellular signal-regulated kinase (ERK) signaling using the ERK inhibitor U0126 and the calcium-channel blocker ketamine inhibited the upregulation of miR-21 expression and MMP9 protein level after cerebral ischemia. The study suggests that cerebral ischemia up-regulates expression level of miR-21, which is involved in ERIC-stimulated upregulation of MMP9 following cerebral ischemia via a calcium-dependent mechanism. (C) 2013 Elsevier Inc. All rights reserved.
