Antibacterial effects of carbon dots in combination with other antimicrobial reagents.
Antibacterial effects of carbon dots in combination with other antimicrobial reagents.
复制标题
DOI：
10.1371/journal.pone.0185324
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Dong X;Awak MA;Tomlinson N;Tang Y;Sun YP;Yang L
本研究旨在研究 CDots 与其他抗菌试剂（包括 H2O2、Na2CO3 和 AcOH（乙酸））结合使用的抗菌效果。合成 CDots 并用 2,2'-(乙撑二氧基)双(乙胺) (EDA) 钝化。 CDots 对革兰氏阴性菌大肠杆菌细胞和革兰氏阳性菌枯草芽孢杆菌细胞的最低抑菌浓度 (MIC) 均为 64 μg/mL。当CDots与H2O2结合时，根据抑制浓度分数（FIC）指数观察到抗菌协同效应，并通过等辐射线图分析和活细胞计数方法进一步证实。 10 μg/mL CDots 与 8.82 mM H2O2 联合处理后，活大肠杆菌细胞数量减少了 2.46 log，显着低于单独使用 8.82 mM H2O2 (1.57 log) 或 10 μg/mL CDots (0.14 log) 处理的对数减少量。然而，CDots与Na2CO3或AcOH的组合并没有表现出协同效应，而是根据FIC指数表现出无差异效应。这项研究表明，CDots与其协同抗菌试剂（例如H2O2）的组合，可以通过使用组合中每种化学物质的浓度低于单独使用一种化学处理的浓度来达到抑制细菌生长的目标，降低对环境健康造成的风险以及微生物产生耐药性的可能性。
This study was designed to investigate the antimicrobial effects of CDots in combination with other antimicrobial reagents, including H2O2, Na2CO3, and AcOH (acetic acid). CDots were synthesized and passivated with 2,2’-(ethylenedioxy)bis(ethylamine) (EDA). The minimal inhibitory concentration (MIC) of CDots was 64 μg/mL on both Gram negative bacteria E.coli cells and Gram positive bacteria Bacillus subtilis cells. When CDots were combined with H2O2, antibacterial synergistic effects were observed based on the fractional inhibitory concentration (FIC) index, and further confirmed by an isobologram analysis and viable cell number counting methods. With the combination treatment of 10 μg/mL CDots with 8.82 mM H2O2, the viable E.coli cell numbers decreased 2.46 log, which was significant lower than the log reduction from 8.82 mM H2O2 (1.57 log) or 10 μg/mL CDots (0.14 log) treatment alone. However, the combination of CDots with Na2CO3 or AcOH did not show synergistic effects, instead, exhibiting indifference effects according to the FIC index. This study indicated that the combination of CDots with their synergistic antimicrobial reagents, such as H2O2, could reach the goal of inhibiting bacteria growth by using lower concentration of each individual chemical in the combination than using one chemical treatment alone, reduce the risks imposed on environmental health and the possibilities of the development of microbial resistances.
登录
查看更多内容
DOI：
10.1083/jcb.93.1.97
发表时间：
1982-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fujiki Y;Hubbard AL;Fowler S;Lazarow PB
通讯作者：
Lazarow PB
碳“量子”点的可见光激活杀菌功能。
影响因子：
9.5
作者：
Meziani, Mohammed J.;Dong, Xiuli;Zhu, Lu;Jones, Les P.;LeCroy, Gregory E.;Yang, Fan;Wang, Shengyuan;Wang, Ping;Zhao, Yiping;Yang, Liju;Tripp, Ralph A.;Sun, Ya-Ping
通讯作者：
Sun, Ya-Ping
影响因子：
2.8
作者：
Baldeck, Jeremiah D.;Marquis, Robert E.
通讯作者：
Marquis, Robert E.
DOI：
10.1046/j.1432-1327.2000.01296.x
发表时间：
2000-05-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Molloy, MP;Herbert, BR;Gooley, AA
通讯作者：
Gooley, AA
影响因子：
2.4
作者：
Murdock, C. A.;Cleveland, J.;Chikindas, M. L.
通讯作者：
Chikindas, M. L.