Analysis of the genetic information of a DNA segment of a new virus from silkworm
Analysis of the genetic information of a DNA segment of a new virus from silkworm
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DOI：
10.1007/bf01315423
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发表时间：
2005
影响因子：
2.7
通讯作者：
H. Bando;T. Hayakawa;S. Asano;K. Sahara;M. Nakagaki;T. Iizuka
中科院分区：
文献类型：
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作者：
H. Bando;T. Hayakawa;S. Asano;K. Sahara;M. Nakagaki;T. Iizuka
1983年，一种细小样病毒（山梨分离物）从家蚕中分离出来。然而，与细小病毒不同，两个DNA分子（VD1和2）总是从纯化的病毒粒子中提取。为了研究病毒基因组的结构和组织，我们确定了VD2的完整核苷酸序列。该序列由6031个核苷酸（nt）组成，包含一个3513个核苷酸的开放阅读框架（ORF 1）。在互补序列中发现一个较小的开放阅读框（ORF 2），长度为702 nt。计算机分析显示，这两个ORF都不编码主要的结构蛋白（VP1，2，3和4）。这些结果表明，VD2本身没有足够的信息来产生子代病毒体。此外，还通过计算机分析预测了VD1和VD2共同的末端序列（CTS）的结构重要性。
In 1983, a parvo-like virus (Yamanashi isolate) was newly isolated from silkworm. However, unlike parvovirus, two DNA molecules (VD1 and 2) were always extracted from purified virions. To investigate the structure and organization of the virus genomes, we determined the complete nucleotide sequence of VD2. The sequence consisted of 6031 nucleotides (nts) and contained a large open reading frame (ORF1) with 3513 nts. A smaller open reading frame (ORF2) with 702 nts was found in the complementary sequence. Computer analysis revealed that both ORFs did not code for the major structural proteins (VP1, 2, 3, and 4). These results suggest that VD2 has not enough information to produce progeny virions by itself. Further, the structural importance of the terminal sequence (CTS) common to both VD1 and VD2 was also predicted by a computer analysis.