Genetic analysis of Giardia from hoofed farm animals reveals artiodactyl-specific and potentially zoonotic genotypes
Genetic analysis of Giardia from hoofed farm animals reveals artiodactyl-specific and potentially zoonotic genotypes
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DOI：
10.1111/j.1550-7408.1997.tb05970.x
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发表时间：
1997-11-01
影响因子：
2.2
通讯作者：
Mayrhofer, G
中科院分区：
文献类型：
--
作者：
Ey, PL;Mansouri, M;Mayrhofer, G
31个贾第虫分离株，建立从6种有蹄类牲畜的无菌培养或生长在乳鼠，遗传比较分析的DNA扩增位点编码的变异表面蛋白或酶谷氨酸脱氢酶和等位酶分析。分离株是异质的，但都表现出亲和力与遗传组合A-两个主要的组合之一，以前定义的分析贾第鞭毛虫从人类。三种不同的基因型是显而易见的。从羊驼、猪、马、牛和羊中分离的10株G.属于先前指定的遗传组（组I）的一种哺乳动物。这种基因型似乎具有广泛的宿主特异性，包括对人类的人畜共患病潜力。来自羊驼、马和羊的5株分离株（2株纯菌和3株乳鼠分离株）与人源性I群和II群G具有密切的亲和力。基因型。其他16个分离株（包括来自澳大利亚和欧洲的牛、绵羊、羊驼、山羊和猪的纯培养物和乳鼠繁殖培养物）与所有其他已通过这些方法检查的具有“鞭毛虫”形态的贾第虫不同，它们作为遗传组合A内的高度不同的亚系（本文称为“新牲畜”）分离。“新型家畜”基因型在试验组中的优势及其与偶蹄动物宿主的明显排他性关联表明，它们可能仅限于这组哺乳动物。在人类和其他一些动物物种中普遍存在的集合B基因型未被检测到。
Thirty one Giardia isolates, established from six species of hoofed livestock by axenic culture or growth in suckling mice, were compared genetically by analysis of DNA amplified from loci encoding variant surface proteins or the enzyme glutamate dehydrogenase and by allozyme analysis. The isolates were heterogeneous, but all showed affinity with genetic Assemblage A-one of two major assemblages defined previously by analysis of Giardia from humans. Three distinct genotypes were evident. Ten isolates (eight axenic and two established in suckling mice) from an alpaca, pig, horse, cattle and sheep were indistinguishable from human-derived G. intestinalis belonging to a previously designated genetic group (Group I). This genotype seems to have broad host specificity, including a zoonotic potential for humans. Five isolates (two axenic and three established in suckling mice) from an alpaca, a horse and sheep had close affinity with human-derived Group I and Group II G. intestinalis genotypes. The other 16 isolates (comprising both axenic and suckling mouse-propagated cultures derived from cattle, sheep, alpaca, a goat and pigs in Australia and Europe) differed from all other Giardia with "duodenalis" morphology that have been examined by these methods and they segregated as a highly distinct sublineage (referred to herein as 'Novel livestock') within genetic Assemblage A. The predominance of 'Novel livestock' genotypes in the test panel and their apparent exclusive association with artiodactyl hosts indicates that they may be confined to this group of mammals. Assemblage B genotypes, which are prevalent in humans and some other animal species, were not detected.