Oxygen Atom-Concentrating Short Fibrous Sponge Regulates Cellular Respiration for Wound Healing
Oxygen Atom-Concentrating Short Fibrous Sponge Regulates Cellular Respiration for Wound Healing
复制标题
DOI：
10.1007/s42765-023-00310-7
复制
发表时间：
2023-07
影响因子：
16.1
通讯作者：
Xiaoyun Fu;Juan Wang;D. Qian;Linhe Xi;Liang Chen;Yawei Du;W. Cui;Yan Wang
中科院分区：
文献类型：
--
作者：
Xiaoyun Fu;Juan Wang;D. Qian;Linhe Xi;Liang Chen;Yawei Du;W. Cui;Yan Wang
细胞呼吸可以为伤口愈合提供能量。然而，在局部高血糖环境中，一些延迟的愈合过程会导致细胞对氧气的适应能力降低，从而降低原位氧化代谢。为此，制备了一种三维(3D)细胞外基质(ECM)仿生短纤维海绵，通过增强细胞对氧气的适应和重塑局部组织微环境，有效地调节了细胞的呼吸。三维仿生海绵支架具有良好的细胞粘附性、生物相容性和生物活性，最重要的是，氧原子聚集在氧化石墨烯(GO)表面。在体外实验中，富氧短纤维海绵可激活单核细胞趋化蛋白-1(MCP-1)，诱导血管内皮生长因子(VEGF)的表达，并有效地促进高血糖环境下的血管生成。该海绵还应用于体内糖尿病创面，以验证其在促进血管生成和胶原沉积方面的作用。这些实验证实了GO与脂肪干细胞(ADSCs)的协同作用，进一步促进了糖尿病伤口的愈合。因此，富含氧原子的短纤维海绵通过改善氧化代谢为修复不良愈合创面提供了新的思路，具有重要的临床意义。图形摘要：聚集氧原子的短纤维海绵的富氧特性可以优化短纤维的吸水性能，通过提高细胞对氧的适应能力来有效调节细胞呼吸，改善组织氧化代谢，为与ADSCs协同进行组织损伤修复提供重要的应用价值。
Cellular respiration can provide energy for wound healing. However, some of retarded healing processes in local hyperglycemic environment suffer from a decrease in cellular adaptation to oxygen, thus reducing in situ oxidative metabolism. Herein, a three-dimensional (3D) extracellular matrix (ECM) bionic short fibrous sponge was prepared for chronic diabetic wound healing and effectively regulated cellular respiration by enhancing cellular adaptation to oxygen and remolding the local tissue microenvironment. The 3D bionic sponge scaffold exhibited good cell adhesion, biocompatibility, bioactivity, and, most importantly, aggregated oxygen atoms on the graphene oxide (GO) surface. In an in vitro assay, the oxygen atom-concentrating short fibrous sponge activated monocyte chemoattractant protein-1 (MCP-1), induced the expression of vascular endothelial growth factor (VEGF), and effectively promoted angiogenesis in a hyperglycemic environment. The sponge was also applied to diabetic wounds in vivo to verify its roles in the promotion of angiogenesis and collagen deposition. These experiments confirmed the synergistic effect of GO with adipose-derived stem cells (ADSCs), which could further promote diabetic wound healing. Therefore, oxygen atom-concentrating short fibrous sponges that regulate cellular respiration provide a new idea for the repair of poorly healing wounds by improving oxidative metabolism and have importantclinical significance.Graphical abstractThe oxygen-rich characteristics of short fibrous sponges with aggregational oxygen atoms can optimize the water absorption performance of short fibers, effectively regulate cell respiration and improve tissue oxidation metabolism by improving the ability of cells to adapt to oxygen, and provide important application value for tissue damage repair in collaboration with ADSCs.