The dependence of radioimmunoassay detection limits on antibody affinity
The dependence of radioimmunoassay detection limits on antibody affinity
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DOI：
10.1016/s0022-1759(97)00148-8
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发表时间：
1997-10-27
影响因子：
2.2
通讯作者：
Gosling, JP
中科院分区：
文献类型：
--
作者：
O'Connor, T;Gosling, JP
用氚标记的孕酮和6种不同的抗孕酮单克隆抗体建立了孕酮的竞争性放射免疫测定标准曲线，其亲和力范围为1.23 × 10 ~（10）~ 2.82 × 10 ~（10）1/mol。这些曲线的检测限范围为133至6，670 pmol/l最终测定浓度（16.7至839.1 pg/管）。单独地，质量作用数学模型用于预测在等效条件下使用六种抗体运行的测定的检测下限。当与实验数据相比时，预测对于较高亲和力抗体是可靠的，但随着亲和力降低而变得不太准确。(C)1997年Elsevier Science B.V.
Competitive radioimmunoassay standard curves for progesterone were established with tritiated progesterone and six different anti-progesterone monoclonal antibodies ranging in affinity from 1.23 X 10(8) to 2.82 X 10(10) 1/mol. The detection limits for these curves ranged from 133 to 6,670 pmol/l final assay concentration (16.7 to 839.1 pg/tube). Separately, a mass-action mathematical model was used to predict lower detection limits for assays run under equivalent conditions with the six antibodies. When compared to the experimental data the predictions were reliable for the higher affinity antibodies but became less accurate as the affinity decreased. (C) 1997 Elsevier Science B.V.