Cell type-specific long-range connections of basal forebrain circuit
Cell type-specific long-range connections of basal forebrain circuit
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DOI：
10.7554/elife.13214
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发表时间：
2016-09-19
期刊：
影响因子：
7.7
通讯作者：
Dan, Yang
中科院分区：
文献类型：
--
作者：
Do, Johnny Phong;Xu, Min;Dan, Yang
基底前脑（BF）在多种脑功能中起着关键作用，包括睡眠-觉醒调节、注意和学习/记忆，但介导这些功能的长程连接仍然很难表征。在这里，我们进行了全脑映射的输入和输出的四个BF细胞类型-胆碱能，谷氨酸能，和小白蛋白阳性（PV+）和生长抑素阳性（SOM+）GABA能神经元-在小鼠大脑。使用狂犬病病毒介导的单突触逆行追踪标记的输入和腺相关病毒追踪轴突投射，我们确定了许多脑区连接到BF。不同细胞类型的输入在性质上是相似的，但输出预测显示出显着差异。与胆碱能神经元和PV+神经元的连接是单向的，而与多巴胺能神经元和SOM+神经元的连接是双向的。这些结果揭示了具有高度收敛输入和发散输出的BF电路的长程布线图，并指出了不同BF细胞类型的功能共性和专业化。
The basal forebrain (BF) plays key roles in multiple brain functions, including sleep-wake regulation, attention, and learning/memory, but the long-range connections mediating these functions remain poorly characterized. Here we performed whole-brain mapping of both inputs and outputs of four BF cell types - cholinergic, glutamatergic, and parvalbumin-positive (PV+) and somatostatin-positive (SOM+) GABAergic neurons - in the mouse brain. Using rabies virus mediated monosynaptic retrograde tracing to label the inputs and adeno-associated virus to trace axonal projections, we identified numerous brain areas connected to the BF. The inputs to different cell types were qualitatively similar, but the output projections showed marked differences. The connections to glutamatergic and SOM+ neurons were strongly reciprocal, while those to cholinergic and PV+ neurons were more unidirectional. These results reveal the long-range wiring diagram of the BF circuit with highly convergent inputs and divergent outputs and point to both functional commonality and specialization of different BF cell types.