Identification of necrotic cell death by the TUNEL assay in the hypoxic-ischemic neonatal rat brain
Identification of necrotic cell death by the TUNEL assay in the hypoxic-ischemic neonatal rat brain
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DOI：
10.1016/s0304-3940(97)00445-x
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发表时间：
1997-07-11
影响因子：
2.5
通讯作者：
Macaya, A
中科院分区：
文献类型：
--
作者：
deTorres, C;Munell, F;Macaya, A
用末端转移酶介导的生物素dUTP缺口末端标记法（TUNEL）检测新生大鼠单侧缺氧缺血模型DNA断裂的时程和定位。损伤后立即在结扎同侧半球中检测到TUNEL阳性细胞，并在1-3天后增加至最大值，然后减少直至第10天，与通过标准组织学方法鉴定的细胞死亡平行。标记显示染色质凝聚和断裂的任何不同形态的细胞，特别是在缺血性病变的核心内。这些结果，在一个范例中获得的坏死在未成熟的大脑，添加到以前的证据表明，某些形式的非凋亡DNA片段标记的TUNEL检测。(C)1997 Elsevier Science爱尔兰有限公司
The time course and localization of DNA fragmentation in a neonatal rat model of unilateral hypoxia-ischemia were assessed by means of the terminal transferase-mediated biotin dUTP nick end labeling (TUNEL) assay. TUNEL-positive cells were detected in the hemisphere ipsilateral to the ligation immediately after the injury and increased to reach a maximum 1-3 days later, then decreasing until day 10, in parallel with cell death identified by standard histological methods. Cells showing any of the different morphologies of chromatin condensation and fragmentation were labeled, particularly within the core of the ischemic lesion. These results, obtained in a paradigm of necrosis in the immature brain, add to previous evidence suggesting that some forms of non-apoptotic DNA fragmentation are labeled by the TUNEL assay. (C) 1997 Elsevier Science Ireland Ltd.