Surface-Engineered Gold Nanorods: Promising DNA Vaccine Adjuvant for HIV-1 Treatment
Surface-Engineered Gold Nanorods: Promising DNA Vaccine Adjuvant for HIV-1 Treatment
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表面工程金纳米棒：有望成为治疗 HIV-1 的 DNA 疫苗佐剂
DOI：
10.1021/nl300027p
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发表时间：
2012-04-01
期刊：
影响因子：
10.8
通讯作者：
Chen, Chunying
中科院分区：
文献类型：
--
作者：
Xu, Ligeng;Liu, Ye;Chen, Chunying
随着国际社会对艾滋病大流行的强烈反应，艾滋病毒疫苗已被广泛研究，但由于人类的安全性或有效性问题而失败。艾滋病毒/艾滋病疫苗的佐剂正在紧张的研究中，但仍然缺乏合理的设计方法。纳米材料由于其独特的物理化学性质，在这一领域代表了一个明显的机会。金纳米结构正被积极研究作为一个有前途的和多功能的平台，用于生物医学应用。在此，我们报告了一种新的表面工程金纳米棒（NR）作为有前途的DNA疫苗佐剂用于艾滋病毒治疗。我们利用表面化学对金纳米棒的佐剂活性的影响，通过放置三种分子，即十六烷基三甲基溴化铵（CTAB），聚（二烯丙基二甲基氯化铵）（PDDAC），和聚乙烯亚胺（PEI）的纳米棒的表面上。如果与体内裸HIV-1 Env质粒DNA处理相比，这些PDDAC-或PEI-修饰的Au NR可以通过活化抗原呈递细胞显著促进细胞和体液免疫以及T细胞增殖。这些发现揭示了低毒纳米材料作为疫苗纳米佐剂/递送系统的通用平台的合理设计。
With the intense international response to the AIDS pandemic, HIV vaccines have been extensively investigated but have failed due to issues of safety or efficacy in humans. Adjuvants for HIV/AIDS vaccines are under intense research but a rational design approach is still lacking. Nanomaterials represent an obvious opportunity in this field due to their unique physicochemical properties. Gold nanostructures are being actively studied as a promising and versatile platform for biomedical application. Herein, we report novel surface-engineered gold nanorods (NRs) used as promising DNA vaccine adjuvant for HIV treatment. We have exploited the effects of surface chemistry on the adjuvant activity of the gold nanorod by placing three kinds of molecules, that is, cetyltrimethylammonium bromide (CTAB), poly(diallydimethylammonium chloride) (PDDAC), and polyethyleneimine (PEI) on the surface of the nanorod. These PDDAC- or PEI-modified Au NRs can significantly promote cellular and humoral immunity as well as T cell proliferation through activating antigen-presenting cells if compared to naked HIV-1 Env plasmid DNA treatment in vivo. These findings have shed light on the rational design of low-toxic nanomaterials as a versatile platform for vaccine nanoadjuvants/delivery systems.