Naringin protects acrolein-induced pulmonary injuries through modulating apoptotic signaling and inflammation signaling pathways in mice
Naringin protects acrolein-induced pulmonary injuries through modulating apoptotic signaling and inflammation signaling pathways in mice
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DOI：
10.1016/j.jnutbio.2018.05.012
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发表时间：
2018-09-01
影响因子：
5.6
通讯作者：
Lee, Jin Hyup
中科院分区：
文献类型：
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作者：
Kim, Jae Kyeom;Park, Jung Hyun;Lee, Jin Hyup
丙烯醛（2-丙烯醛）在环境中普遍存在，丙烯醛暴露与肺癌风险之间存在联系。本文研究了柚苷对丙烯醛所致小鼠肺损伤的影响。将雄性C57 BL/6小鼠分成四组：媒介物组（无丙烯醛）、仅柚苷组（80 mg柚苷/kg bw +无丙烯醛）、丙烯醛组（ACR组;丙烯醛）和柚苷+丙烯醛组（NAG+ACR组; 80 mg柚苷/kg bw和丙烯醛）。在吸入室中对小鼠进行急性丙烯醛吸入（10 ppm，持续12小时），并在丙烯醛暴露前1小时向小鼠腹膜内施用柚皮苷。结果表明，在NAG+ACR组中，肺损伤（例如，与ACR组相比，所有患者的肺功能均得到显著改善。此外，MAPK信号传导的关键标志物（例如，p-p38，p-JNK），p53信号传导标志物（例如，p-Chk 2，p53），NF-κ B信号传导轴（例如，IL-1 β、TNF-α）和氧化损伤标志物（例如，GSSG：GSH比值、氧化性DNA损伤）均被柚苷处理有效地减轻。柚皮苷通过调节MAPK、p53和NF-κ B B信号通路，在小鼠肺中提供对环境毒物丙烯醛的保护，我们的数据可能提供考虑丙烯醛流行的重要意义。(C)2018由Elsevier Inc.出版
Acrolein (2-propenal) is ubiquitous in the environment and connections exist between acrolein exposure and lung cancer risk. Here we investigated the effects of naringin on acrolein induced-lung injuries in mice. Male C57BL/6 mice were allocated into four groups: Vehicle group (no acrolein), Naringin only group (80 mg of naringin/kg bw + no acrolein), Acrolein group (ACR group; acrolein), and Naringin + Acrolein group (NAG+ACR group; 80 mg of naringin/kg bw and acrolein). The mice were subjected acute acrolein inhalation (10 ppm for 12 h) in an inhalation chamber and naringin was intraperitoneally administered to the mice one hour before acrolein exposure. The results demonstrated that, in the NAG+ACR group, pulmonary injuries (e.g., airspace enlargement, lung inflammation) were all significantly improved compared to the ACR group. Further, key markers of MAPK signaling (e.g., p-p38, p-JNK), p53 signaling markers (e.g., p-Chk2, p53), NF-kappa B signaling axis (e.g., IL-1 beta, TNF-alpha), and oxidative damage markers (e.g., GSSG:GSH ratio, oxidative DNA damage) were all effectively mitigated by the naringin treatment. Naringin provided protection against the environmental toxicant, acrolein, in mice lung via modulating MAPK, p53, and NF-kappa B signaling pathways and our data may provide significant implications considering the prevalence of acrolein. (C) 2018 Published by Elsevier Inc.