Differences in the fatty-acid composition of rodent spermatozoa are associated to levels of sperm competition.
Differences in the fatty-acid composition of rodent spermatozoa are associated to levels of sperm competition.
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DOI：
10.1242/bio.201411288
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发表时间：
2015-03-20
期刊：
影响因子：
2.4
通讯作者：
Roldan ER
中科院分区：
文献类型：
--
作者：
delBarco-Trillo J;Mateo R;Roldan ER
精子竞争是推动精子功能进化的普遍现象。高水平的精子竞争导致新陈代谢加快，从而提高精子的速度。这种增强的代谢可导致氧化损伤（包括脂质过氧化）和膜损伤。我们假设，在那些经历高水平精子竞争的物种中，精子膜的脂肪酸组成发生了变化，使膜更能抵抗氧化损伤。鉴于多不饱和脂肪酸（PUFAs）最容易发生脂质过氧化，我们预测精子竞争加剧会导致精子PUFAs比例降低。相反，我们预测精子竞争水平不应影响体细胞中PUFAs的比例。为了验证这些预测，我们对精子竞争水平不同的四种小鼠（鼠属）的精子、睾丸和肝细胞的脂肪酸组成进行了量化。睾丸和肝细胞中的脂肪酸组成与精子竞争水平无关。然而，在精子细胞中，正如预测的那样，精子竞争水平的增加与饱和脂肪酸（最抗脂质过氧化）比例的增加以及pufa比例的减少有关。两种特殊的脂肪酸（花生四烯酸和棕榈酸）是这种模式的主要原因。因此，我们的研究结果表明，精子竞争对精子细胞的组成具有普遍的影响，最终可能对精子功能产生重要影响。
Sperm competition is a prevalent phenomenon that drives the evolution of sperm function. High levels of sperm competition lead to increased metabolism to fuel higher sperm velocities. This enhanced metabolism can result in oxidative damage (including lipid peroxidation) and damage to the membrane. We hypothesized that in those species experiencing high levels of sperm competition there are changes in the fatty-acid composition of the sperm membrane that makes the membrane more resistant to oxidative damage. Given that polyunsaturated fatty acids (PUFAs) are the most prone to lipid peroxidation, we predicted that higher sperm competition leads to a reduction in the proportion of sperm PUFAs. In contrast, we predicted that levels of sperm competition should not affect the proportion of PUFAs in somatic cells. To test these predictions, we quantified the fatty-acid composition of sperm, testis and liver cells in four mouse species (genus Mus) that differ in their levels of sperm competition. Fatty-acid composition in testis and liver cells was not associated to sperm competition levels. However, in sperm cells, as predicted, an increase in sperm competition levels was associated with an increase in the proportion of saturated fatty-acids (the most resistant to lipid peroxidation) and by a concomitant decrease in the proportion of PUFAs. Two particular fatty acids were most responsible for this pattern (arachidonic acid and palmitic acid). Our findings thus indicate that sperm competition has a pervasive influence in the composition of sperm cells that ultimately may have important effects in sperm function.
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DOI：
10.1073/pnas.0809990106
发表时间：
2009-01-27
影响因子：
11.1
作者：
Fitzpatrick, John L.;Montgomerie, Robert;Balshine, Sigal
通讯作者：
Balshine, Sigal
DOI：
10.1073/pnas.0605795103
发表时间：
2006-10-10
影响因子：
11.1
作者：
Gomendio, Montserrat;Martin-Coello, Juan;Roldan, Eduardo R. S.
通讯作者：
Roldan, Eduardo R. S.
影响因子：
2.1
作者：
Firman, R. C.;Simmons, L. W.
通讯作者：
Simmons, L. W.
影响因子：
2.1
作者：
delBarco-Trillo, J.
通讯作者：
delBarco-Trillo, J.
影响因子：
4
作者：
Breitbart, H;Spungin, B
通讯作者：
Spungin, B