Exposure to cadmium causes inhibition of otolith development and behavioral impairment in zebrafish larvae
Exposure to cadmium causes inhibition of otolith development and behavioral impairment in zebrafish larvae
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接触镉会抑制斑马鱼幼虫的耳石发育和行为障碍
DOI：
10.1016/j.aquatox.2019.105236
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发表时间：
2019-09-01
影响因子：
4.5
通讯作者：
Zhou, Bingsheng
中科院分区：
文献类型：
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作者：
Han, Jian;Liu, Kechun;Zhou, Bingsheng
耳石主要由碳酸钙、纤维和蛋白质组成，对维持鱼类的身体平衡和/或听觉至关重要。耳石的形成涉及Ca ~（2+）的转运和沉积。以斑马鱼胚胎为模型，研究了Cd ~（2+）对斑马鱼耳石发育的影响。结果表明，暴露于Cd ~（2+）抑制椭圆囊和囊状耳石的生长，表现为侧面积减少。Cd ~（2+）暴露的幼虫游泳速度降低，失去平衡控制。与Ca 2+转运相关的基因（例如质膜Ca 2 + -ATP酶亚型2，pmca 2; Ca 2 + -ATP酶亚型2，atp 2b 1a）和调节相关的基因（例如甲状旁腺激素配体1型，pth 1;斯钙素亚型1，stc 1）显著下调。但在暴露液中添加Ca ~（2+）可保护Cd ~（2+）对耳石生长和游泳活动的不利影响。补充Ca ~（2+）可降低仔鱼体内Cd ~（2+）的负荷。结果表明，Cd ~（2+）暴露可抑制Ca ~（2+）内流，导致耳石生长所需的CaCO_3沉积减少，最终导致斑马鱼幼鱼平衡控制和游泳能力下降。
Otolith consisting largely of calcium carbonate, fibrous and proteins, is vital for maintaining body balance and/or hearing of fish. The formation of otolith involves Ca2+ transport and deposition. In the present study, we investigated the effects of Cd2+ on otoliths development by using zebrafish embryos as model. The results showed that exposure to Cd2+ inhibited the utricular and saccular otoliths growth, indicated by reduced lateral areas. Swimming speeds were reduced and a losing balance control was observed in Cd2+ exposed larvae. The genes related to Ca2+ transport (e.g. plasma membrane Ca2+ -ATPase isoform 2, pmca2; Ca2+ -ATPase isoform 2, atp2b1a) and regulation (e.g. parathyroid hormone ligand type-1, pth1; stanniocalcin isoform 1, stc1) were significantly downregulated. However, the adverse effects of Cd2+ on otoliths growth and swimming activity can be protected by supplementation of Ca2+ in exposure medium. Body burden of Cd2+ in larvae was reduced upon the supplement with Ca2+. The overall results suggest that exposure to Cd2+ can inhibit influx of Ca2+, leading to less deposition of CaCO3 for otolith growth, and finally result in impaired balance control and swimming activity in zebrafish larvae.