TMEM158 May Serve as a Diagnostic Biomarker for Anaplastic Thyroid Carcinoma: An Integrated Bioinformatic Analysis
TMEM158 May Serve as a Diagnostic Biomarker for Anaplastic Thyroid Carcinoma: An Integrated Bioinformatic Analysis
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TMEM158 可作为甲状腺未分化癌的诊断生物标志物：综合生物信息学分析
DOI：
10.1007/s11596-020-2296-8
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发表时间：
2020-12-01
影响因子：
2.4
通讯作者：
Li, Xing-rui
中科院分区：
文献类型：
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作者：
Li, Han-ning;Du, Ya-ying;Li, Xing-rui
甲状腺未分化癌是一种罕见但致命性极高的恶性肿瘤。然而，对ATC的发病机制知之甚少。鉴于其高死亡率，提高我们对ATC发病机制的理解并找到新的诊断生物标志物至关重要。在本研究中，两个基因芯片（GSE 53072和GSE 65144），其中包括17个ATC和17个相邻的非肿瘤组织，获得了。然后进行生物信息学分析。然后使用免疫组织化学（IHC）和受试者工作特征（ROC）曲线检测跨膜蛋白158（TMEM 158）表达并评估诊断灵敏度。共鉴定出372个差异表达基因（DEG）。通过蛋白质-蛋白质相互作用（PPI）分析，我们确定了一个有37个上调基因的重要模块。该模块中的大多数基因与细胞周期过程有关。共表达分析后，选择了132个枢纽基因进行进一步研究。在加权基因共表达网络分析（WGCNA）中，9个基因被鉴定为DEG和感兴趣的基因。IHC和ROC曲线证实，与其他类型的甲状腺癌和正常组织样品相比，TMEM 158在ATC组织中过表达。我们鉴定了8条与TMEM 158高表达相关的KEGG通路，包括氨酰-tRNA生物合成和DNA复制。我们的研究结果表明，TMEM 158可能是一个潜在的癌基因，并作为ATC的诊断指标。
Anaplastic thyroid carcinoma (ATC) is a rare but extremely lethal malignancy. However, little is known about the pathogenesis of ATC. Given its high mortality, it is critical to improve our understanding of ATC pathogenesis and to find new diagnostic biomarkers. In the present study, two gene microarray profiles (GSE53072 and GSE65144), which included 17 ATC and 17 adjacent non-tumorous tissues, were obtained. Bioinformatic analyses were then performed. Immunohistochemistry (IHC) and receiver operating characteristic (ROC) curves were then used to detect transmembrane protein 158 (TMEM158) expression and to assess diagnostic sensitivity. A total of 372 differentially expressed genes (DEGs) were identified. Through protein-protein interaction (PPI) analysis, we identified a significant module with 37 upregulated genes. Most of the genes in this module were related to cell-cycle processes. After co-expression analysis, 132 hub genes were selected for further study. Nine genes were identified as both DEGs and genes of interest in the weighted gene co-expression network analysis (WGCNA). IHC and ROC curves confirmed that TMEM158 was overexpressed in ATC tissue as compared with other types of thyroid cancer and normal tissue samples. We identified 8 KEGG pathways that were associated with high expression of TMEM158, including aminoacyl-tRNA biosynthesis and DNA replication. Our results suggest that TMEM158 may be a potential oncogene and serve as a diagnostic indicator for ATC.