A rapid and effective nonsurgical artificial insemination protocol using the NSET™ device for sperm transfer in mice without anesthesia.
A rapid and effective nonsurgical artificial insemination protocol using the NSET™ device for sperm transfer in mice without anesthesia.
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DOI：
10.1007/s11248-015-9887-3
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发表时间：
2015-08
影响因子：
3
通讯作者：
Fath-Goodin A
中科院分区：
文献类型：
--
作者：
Stone BJ;Steele KH;Fath-Goodin A
人工授精（AI）是一种辅助生殖技术，在人类中成功实施，作为生育治疗，广泛用于牲畜的商业繁殖，在实验室啮齿动物中也很成功。小鼠中的AI可能特别适用于繁殖具有生育问题的转基因或突变小鼠，扩大小鼠集落，并作为体外受精的替代方法。之前已经描述了用于小鼠的非手术AI技术，但由于技术困难而不经常实施。在这里，我们比较了各种方案的准备CD1受体前AI天真（在发情期），排卵诱导，超排卵的女性。还比较了激素施用相对于精子递送的时间。一种改进的协议，非手术AI小鼠，其中包括一个方便的激素给药计划，为女性受体和快速，无压力的精子移植，而不需要麻醉或镇痛。
Artificial insemination (AI) is an assisted reproductive technique that is implemented successfully in humans as a fertility treatment, performed extensively for commercial breeding of livestock, and is also successful in laboratory rodents. AI in the mouse may be especially useful for breeding of transgenic or mutant mice with fertility problems, expansion of mouse colonies, and as an alternative to in vitro fertilization. Nonsurgical AI techniques for the mouse have been described previously but are not often implemented due to technical difficulties. Here we compare various protocols for preparation of CD1 recipients prior to AI for naïve (in estrus), ovulation-induced, and superovulated females. Timing of hormone administration relative to sperm delivery is also compared. An improved protocol for nonsurgical AI in mice is described, which incorporates a convenient hormone administration schedule for female recipients and rapid, non-stressful sperm transfer without the need for anesthesia or analgesia.