Ethanol has direct inhibitory effects on steroidogenesis in human granulosa cells: specific inhibition of LH action.
Ethanol has direct inhibitory effects on steroidogenesis in human granulosa cells: specific inhibition of LH action.
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DOI：
10.1111/j.1530-0277.1990.tb01192.x
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发表时间：
1990-08
期刊：
影响因子：
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通讯作者：
S. Saxena;D. Meehan;P. Coney;J. Wimalasena
中科院分区：
文献类型：
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作者：
S. Saxena;D. Meehan;P. Coney;J. Wimalasena
我们评价了乙醇(EtoH)对体外受精过程中培养的人颗粒细胞产生孕酮(P)和17-雌二醇(E_2)的直接影响。培养第3天，将细胞分为对照组和乙醇(20 MM)组，分别用hFSH(50 ng/ml)、人促黄体生成素(0-50 ng/ml)、卵泡刺激素+黄体生成素、8-溴-cAMP(0.25 mM)和雄烯二酮(10(-7)M)刺激。实验在第7天和第9天结束，并测定DNA、P和E_2。乙醇可抑制促黄体生成素刺激的P和E_2的分泌，而在对照组和FSH或cAMP刺激下，乙醇对P和E_2的分泌无明显影响。乙醇对雄烯二酮刺激的E_2的产生也没有影响。乙醇组人颗粒细胞DNA含量与对照组比较差异无统计学意义。这些结果首次证实了乙醇对培养的颗粒黄体细胞的直接作用，并提示乙醇可能抑制黄体激素对黄体的作用。酒精对卵巢的直接选择性毒性可能是酗酒女性生殖异常的原因。
We have evaluated the direct effects of ethanol (EtOH) on the production of progesterone (P) and estradiol-17 beta (E2) by cultured human granulosa cells obtained during in vitro fertilization procedures. On day 3 of culture, cells were divided into control and ethanol (20 mM) groups and stimulated by hFSH (50 ng/ml), hLH (0-50 ng/ml), FSH+LH, 8 Br-cAMP (0.25 mM) and androstenedione (10(-7) M). Experiments were terminated on days 7 and 9 and DNA, P, and E2 were measured. Ethanol inhibited P and E2 secretion stimulated by LH; however, there was no significant effect of ethanol on P and E2 production in the control group or when the cells were stimulated by FSH or cAMP. EtOH also had no effect on androstenedione stimulated E2 production. There was no significant difference in the DNA contents of the human granulosa cells in the ethanol group as compared with the control group. These results are the first demonstration of a direct effect of ethanol on cultured granulosa-lutein cells and suggest that ethanol may inhibit action of LH on the corpus luteum. A direct selective toxic effect of EtOH on the ovary may be responsible for some of the reproductive abnormalities observed in alcoholic women.