New artificial nerve conduits made with photocrosslinked hyaluronic acid for peripheral nerve regeneration.
New artificial nerve conduits made with photocrosslinked hyaluronic acid for peripheral nerve regeneration.
复制标题
由光交联透明质酸制成的新型人造神经导管，用于周围神经再生。
DOI：
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发表时间：
2007
影响因子：
1
通讯作者：
N. Ishiguro
中科院分区：
文献类型：
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作者：
Yoshihito Sakai;Y. Matsuyama;Katsuya Takahashi;Tomoya Sato;T. Hattori;S. Nakashima;N. Ishiguro
对于周围神经再生，多孔支架内细胞的三维分布和生长具有临床意义。我们证明，培养的大鼠雪旺细胞和神经球生长在体外新的人工神经导管与光交联透明质酸（HA）。HA管状导管的内径为1.2 mm，具有50 μ m的多孔纳米结构。培养3周后，HA导管保持圆形，具有圆形管腔，并且细胞导管的构建保持了管的原始结构的大小和形状。HA本身具有在周围神经再生期间促进细胞和轴突向内生长的途径的功能。这些发现提供了使用HA导管更好的细胞粘附和分化的可行性，从而导致周围神经重建中的轴突再生。
For peripheral nerve regeneration, three-dimensional distribution and growth of cells within the porous scaffold are of clinical significance. We demonstrate that cultured rat Schwann cells and neurospheres grow in vitro on new artificial nerve conduits made with photocrosslinked hyaluronic acid (HA). HA tubular conduits have an inner diameter of 1.2 mm with porous nano-structure of 50 microm. After 3 weeks of cultivation, HA conduits remained circular with a round lumen, and construct of cell-conduits maintained the size and shape of the original architecture of the tube. HA itself has the function to facilitate a pathway for cellular and axonal ingrowth during peripheral nerve regeneration. These findings provide the feasibility of using the HA conduits for better cell adhesion and differentiation, leading to axonal regeneration in peripheral nerve reconstruction.