Hyaluronic acid/platelet rich plasma-infused core-shell nanofiber membrane to prevent postoperative tendon adhesion and promote tendon healing
Hyaluronic acid/platelet rich plasma-infused core-shell nanofiber membrane to prevent postoperative tendon adhesion and promote tendon healing
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DOI：
10.1016/j.ijbiomac.2023.123312
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发表时间：
2023-01-21
影响因子：
8.2
通讯作者：
Chen, Jyh-Ping
中科院分区：
文献类型：
--
作者：
Chen, Chih-Hao;Chen, Shih-Hsien;Chen, Jyh-Ping
含有透明质酸（HA）的抗粘连屏障膜可以减少成纤维细胞的附着，并在手术后肌腱粘连的治疗过程中为肌腱的平滑滑动提供润滑作用。另一方面，由于肌腱恢复过程中需要大量的生长因子，血小板在富血小板血浆（PRP）中释放的生长因子可以提供有益的治疗作用，促进肌腱损伤后的恢复。此外，据报道，PRP与抑制术后粘连的抗炎特性有关。为此，我们在本研究中制备了HA/ prp注入芯和聚己内酯壳的核壳纳米纤维膜（NFM）。采用同轴静电纺丝法制备了含100% （H-P）、75% （HP31-P）、50% （HP11-P）和25% (H31-P) HA的NFM，并通过显微、孔径、力学、HA和生长因子释放研究对其进行了分析。成纤维细胞的体外研究表明，除了促进肌腱愈合中的肌腱细胞迁移外，NFM还可以作为阻止细胞渗透和减少细胞附着/局灶性粘附的屏障。兔屈肌腱断裂模型的体内研究表明，从功能分析和组织学分析来看，HP11-P NFM在减少肌腱粘连形成和炎症、促进肌腱愈合方面比hp - p NFM具有更好的疗效。
An anti-adhesive barrier membrane incorporating hyaluronic acid (HA) can reduce fibroblasts attachment and impart lubrication effect for smooth tendon gliding during management of post-surgical tendon adhesion. On the other hand, as numerous growth factors are required during tendon recovery, growth factors released by platelets in platelet-rich plasma (PRP) can provide beneficial therapeutic effects to facilitate tendon recovery post tendon injury. Furthermore, PRP is reported to be associated with anti-inflammatory properties for suppressing postoperative adhesion. Toward this end, we fabricate core-shell nanofiber membranes (NFM) with HA/PRP-infused core and polycaprolactone shell in this study. Different NFM with 100 % (H-P), 75 % (HP31-P), 50 % (HP11-P) and 25 % (H31-P) HA in the core was fabricated through coaxial electrospinning and analyzed through microscopic, pore size, mechanical, as well as HA and growth factor release studies. In vitro study with fibroblasts indicates the NFM can act as a barrier to prevent cell penetration and reduce cell attachment/focal adhesion, in addition to promoting tenocyte migration in tendon healing. In vivo studies in a rabbit flexor tendon rupture model indicates the HP11-P NFM shows improved efficacy over H-P NFM and control in reducing tendon adhesion formation and inflammation, while promoting tendon healing, from functional assays and histological analysis.