Identification and potential role of PSD-95 in Schwann cells
Identification and potential role of PSD-95 in Schwann cells
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DOI：
10.1007/s10072-008-0989-z
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发表时间：
2008-10-01
影响因子：
3.3
通讯作者：
Cheng, Chun
中科院分区：
文献类型：
--
作者：
Shen, Aiguo;Gao, Shangfeng;Cheng, Chun
突触后密度-95（Postsynaptic density-95，PSD-95）是神经元型一氧化氮合酶（nNOS）的锚定蛋白，在确定一氧化氮（nitric oxide，NO）在神经系统中的反应位点方面起着重要作用。本研究旨在探讨PSD-95在大鼠雪旺细胞（SC）中的存在以及PSD-95和nNOS与血清诱导的SC增殖的关系。血清剥夺48 h后两种分子的表达均显著下调，并逐渐升高，至12 h达高峰，血清刺激后48 h基本恢复至对照水平。在Ki 67和BrdU阳性的SC中观察到PSD-95与nNOS的关联。选择性nNOS抑制剂可阻滞细胞周期进程，降低增殖细胞核抗原（PCNA）水平。这些结果表明，PSD-95和nNOS可能共同参与了SC的增殖，为NO在周围神经再生中的作用提供了进一步的证据。
Postsynaptic density-95 (PSD-95) is one of neuronal nitric oxide synthase (nNOS)-anchoring proteins and plays an important role in specifying the sites of reaction of nitric oxide (NO) in the nervous system. The present study aims to investigate the presence of PSD-95 in rat Schwann cells (SCs) and the association of PSD-95 and nNOS with serum-induced SCs proliferation. The expression of both molecules downregulated significantly after 48 h of serum deprivation, and increased gradually to the peak at 12 h, ultimately returned to the control level at 48 h after serum stimulation. The association of PSD-95 with nNOS was observed in Ki67 and BrdU-positive SCs. The selective nNOS inhibitor arrested the cell cycle progress and decreased the proliferating cell nuclear antigen (PCNA) levels. These findings suggested that PSD-95 and nNOS may collectively participate in the proliferation of SCs, providing further evidence for the role of NO during peripheral nerve regeneration.