Substrain heterogeneity in prostaglandin E2 synthesis of human dermal fibroblasts. Differences in prostaglandin E2 synthetic capacity of substrains are not stimulus-restricted.
Substrain heterogeneity in prostaglandin E2 synthesis of human dermal fibroblasts. Differences in prostaglandin E2 synthetic capacity of substrains are not stimulus-restricted.
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人真皮成纤维细胞前列腺素 E2 合成的子系异质性。
DOI：
10.1002/art.1780280312
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发表时间：
1985
影响因子：
--
通讯作者：
Korn,JH
中科院分区：
文献类型：
--
作者：
Korn,JH
我们研究了前列腺素E2（PGE 2）的生物合成异质性成纤维细胞亚株和可能的机制，可能介导这种异质性。响应于植物血凝素刺激的单核细胞上清液，成纤维细胞亚株的PGE 2合成范围为9.0 ± 1.0 ng/ml至79.3 ± 7.4 ng/ml（平均值± SD）。各亚株的表型行为稳定。各亚株对佛波酯的反应也具有异质性，并表现出稳定的表型。对单核细胞上清液有高反应的亚株对佛波醇肉豆蔻酸酯的反应也很高。在对纯化白细胞介素-1的反应中观察到相似的异质性。外源性添加花生四烯酸并没有提高低产量亚株对高产量亚株的白细胞介素-1反应性，这表明亚株之间PGE 2合成的差异并不反映底物可用性或磷脂酶活性的差异。高和低反应表型亚株的上清液，当加入到相互菌株的细胞或无关的成纤维细胞中时，不影响PGE 2合成的模式。亚株对单核细胞上清液和佛波醇肉豆蔻酸酯的反应一致性表明，PGE 2合成亚株之间的异质性与产生PGE 2的能力有关，而不是对给定介质的反应能力。此外，亚株间PGE 2合成的差异似乎不是由于调节性自身因子的释放。
We examined prostaglandin E2(PGE2) biosynthetic heterogeneity in fibroblast substrains and possible mechanisms that might mediate this heterogeneity. PGE2synthesis of fibroblast substrains, in response to phytohemagglutinin‐stimulated mononuclear cell supernates, ranged from 9.0 ± 1.0 ng/ml to 79.3 ± 7.4 ng/ml (mean ± SD). The phenotypic behavior of individual substrains was stable. Substrains were also heterogeneous in PGE2response to phorbol myristate acetate, and displayed stability in this phenotype as well. Substrains which were high responders to mononuclear cell supernate also ranked high in response to phorbol myristate acetate. Similar heterogeneity was observed in response to purified interleukin‐1. Arachidonic acid added exogenously did not raise interleukin‐1 responsiveness of low‐producer substrains to that of high producers, suggesting that differences in PGE2synthesis among substrains did not reflect differences in substrate availability or phospholipase activity. Supernates of high‐ and low‐responder phenotype substrains, when added to cells of the reciprocal strain or to unrelated fibroblasts, did not affect the pattern of PGE2synthesis. The concordance of substrain responsiveness to mononuclear cell supernate and phorbol myristate acetate suggests that heterogeneity among substrains in PGE2synthesis is related to the ability to produce PGE2rather than to the ability to respond to a given mediator. In addition, differences in PGE2synthesis among substrains do not appear to result from release of regulatory autokines.