The Effects of Fibroblast Growth Factor-2 on Rotator Cuff Reconstruction With Acellular Dermal Matrix Grafts
The Effects of Fibroblast Growth Factor-2 on Rotator Cuff Reconstruction With Acellular Dermal Matrix Grafts
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DOI：
10.1016/j.arthro.2008.11.011
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发表时间：
2009-06-01
影响因子：
4.7
通讯作者：
Mizuta, Hiroshi
中科院分区：
文献类型：
--
作者：
Ide, Junji;Kikukawa, Kenshi;Mizuta, Hiroshi
目的：我们的目的是确定是否局部应用成纤维细胞生长因子（FGF）2加速再生和重建的肩袖肌腱缺损与脱细胞真皮基质（ADM）移植重建大鼠。方法：30只成年雄性Sprague-Dawley大鼠被分成相等的组，接受FGF治疗和FGF未治疗的修复。所有大鼠均接受所有ADM移植物的放置，用于冈上肌缺损（3 x 5 mm）。FGF-2（100 μ g/kg）在纤维蛋白封闭剂中应用于FGF治疗组的双肩，而在未治疗组中仅应用纤维蛋白封闭剂。术后2、6、12周，每组处死5只大鼠（10个肩关节），进行组织学分析（3个肩关节）和生物力学测试（7个肩关节）。对照组为5只未手术大鼠（3只组织学对照标本和7只生物力学对照标本）。结果：未手术对照肌腱直接插入骨中，肌腱和骨之间有纤维软骨区。在2周时，FGF治疗组的肌腱成熟评分与未治疗组相似（P> 0.05）。在第6周和第12周，FGF治疗组的评分显著更高（P.05）。在6周时，FGF处理的样本更强，极限拉伸失效载荷为10.2 +/- 3.1 N，而未处理组为7.2 +/- 2.2 N（P= 0.02）。在12周时，FGF处理的样本更强，极限拉伸破坏载荷为15.9 +/- 1.6 N，而未处理组为13.2 +/- 2.0 N（P> 0.0072），与对照组（17.8 +/- 2.6 N）相比，强度无显著差异（P> 0.05）。结论：局部应用FGF-2可加速大鼠肩袖肌腱缺损处ADM移植物的重建。
Purpose: Our purpose was to determine whether the local application of fibroblast growth factor (FGF) 2 accelerates regeneration and remodeling of rotator cuff tendon defects reconstructed with acellular dermal matrix (ADM) grafts in rats. Methods: Thirty adult male Sprague-Dawley rats were divided into equal groups undergoing FGF-treated and FGF-untreated repairs. All rats underwent placement of ail ADM graft for the supraspinatus defect (3 x 5 mm). FGF-2 (100 mu g/kg) in a fibrin sealant was applied to both shoulders in the FGF-treated group, whereas only fibrin sealant was applied in untreated group. At 2, 6, and 12 weeks after surgery, 5 rats (10 shoulders) in each group were sacrificed for histologic analysis (3 shoulders) and biomechanical testing (7 shoulders). The controls were 5 unoperated rats (3 histologic and 7 biomechanical control specimens). Results: Unoperated control tendons inserted into the bone by direct insertion; there was a zone of fibrocartilage between the tendon and bone. At 2 weeks, the FGF-treated group had tendon maturing scores similar to those in the untreated group (P>.05). At 6 and 12 weeks, the FGF-treated group had significantly higher scores (P.05). At 6 weeks, the FGF-treated specimens were stronger, with an ultimate tensile failure load of 10.2 +/- 3.1 N compared with 7.2 +/- 2.2 N in the untreated group (P=.02). At 12 weeks, the FGF-treated specimens were stronger, with an ultimate tensile failure load of 15.9 +/- 1.6 N compared with 13.2 +/- 2.0 N in the untreated group (P>.0072), and there were no significant differences in strength compared with the controls (17.8 +/- 2.6 N) (P>.05). Conclusions: The remodeling of ADM grafts placed in rat rotator cuff tendon defects was accelerated by the local administration of FGF-2.