Stepwise Elusion Method in Micellar Electrokinetic Chromatography via Sequential Use of Lithium Perfluorooctadecyl Sulfonate and Lithium Dodecyl Sulfate
Stepwise Elusion Method in Micellar Electrokinetic Chromatography via Sequential Use of Lithium Perfluorooctadecyl Sulfonate and Lithium Dodecyl Sulfate
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依次使用全氟十八烷基磺酸锂和十二烷基硫酸锂的胶束电动色谱中的逐步洗脱法
DOI：
10.1016/j.chroma.2014.06.073
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
B. Uno
中科院分区：
文献类型：
--
作者：
Y. Esaka;F. Rin;M. Kobayashi;R. Osako;H. Murakami;B. Uno
以全氟十八烷基磺酸锂（LPFOS）和十二烷基硫酸锂（LDS）为洗脱剂，建立了一种有效的分步胶束电动色谱（MEKC）洗脱方法。LPFOS胶束的氢键性质不同于LDS胶束，这导致溶质向胶束转移的选择性显著不同。本分步方法是在酸性条件下，在不存在电渗流的情况下，通过将含有LPFOS的第一运行溶液的入口储液器替换为含有LDS的第二运行溶液的入口储液器来进行的，其中LPFOS胶束首先作为载体，然后LDS胶束翻转。15种非离子芳香族化合物的有效分离通过调节入口储液器中的时间得到很好的控制，这在仅使用LPFOS或仅使用LDS的系统中无法实现，在必要时对洗脱顺序进行重大改变。此外，分离与本逐步的方法很容易模拟，和更换时间优化为3.1分钟，从70.0 mM LPFOS溶液到67.5 mM LDS溶液，几乎完全分离在15分钟内使用模拟参数。
An effective stepwise micellar electrokinetic chromatography (MEKC) elution method was developed using lithium perfluorooctadecyl sulfonate (LPFOS) and lithium dodecyl sulfate (LDS). The hydrogen-bonding property of LPFOS micelles differs from that of LDS micelles, which leads to remarkably different selectivity in the transfer of solutes to the micelles. The present stepwise method is performed by replacing the inlet reservoir of a first running solution containing LPFOS with that of a second running solution containing LDS during a single separation run in the absence of electroosmotic flow under acidic conditions, where LPFOS micelles work as carriers in first and then LDS micelles turn over. Effective separation of 15 nonionic aromatic compounds was controlled well by adjusting the time in the inlet reservoir, which could not be accomplished with systems using only LPFOS or only LDS, with significant changes in the elution order where necessary. Furthermore, separations with the present stepwise method were easily simulated, and the replacement time was optimized for 3.1 min from a 70.0 mM LPFOS solution to a 67.5 mM LDS solution with nearly complete separation within 15 min using the simulated parameters.