An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells.
An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells.
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用于快速、高灵敏度分析循环肿瘤细胞的集成微流体装置
DOI：
10.1038/srep42612
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发表时间：
2017-02-15
影响因子：
4.6
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Jiang J;Zhao H;Shu W;Tian J;Huang Y;Song Y;Wang R;Li E;Slamon D;Hou D;Du X;Zhang L;Chen Y;Wang Q
近年来，由于循环肿瘤细胞（CTC）在转移性癌症的预后和治疗中的重要性，开发有效的CTC检测技术受到了更多的关注。然而，这仍然是一个挑战，因为血液中的CTC计数低。本文报道了一种快速、高灵敏度的CTCs检测方法，该方法由确定性侧向位移（DLD）隔离结构、CD 45标记免疫磁珠的自动纯化装置和鼠尾胶原包被的捕获平台组成。通过从血液中捕获绿色荧光蛋白（GFP）阳性细胞，我们观察到在1 mL/min的高通量下90%的高捕获率、约50%的纯度和超过90%的存活率。进一步捕获来自转移性癌症患者的CTC显示阳性捕获率为83.3%。此外，通过对30名癌症患者的平行分析，将我们的设备与CellSearch系统进行了比较，发现两种方法的捕获效率之间没有显着差异。然而，我们的设备在时间、样品量和分析成本方面显示出优势。因此，我们的无菌环境和使用方便的集成设备将是一个有前途的平台，具有潜在的临床应用的CTC检测。
Recently there has been a more focus on the development of an efficient technique for detection of circulating tumor cells (CTCs), due to their significance in prognosis and therapy of metastatic cancer. However, it remains a challenge because of the low count of CTCs in the blood. Herein, a rapid and high-sensitivity approach for CTCs detection using an integrated microfluidic system, consisting of a deterministic lateral displacement (DLD) isolating structure, an automatic purifying device with CD45-labeled immunomagnetic beads and a capturing platform coated with rat-tail collagen was reported. We observed high capture rate of 90%, purity of about 50% and viability of more than 90% at the high throughput of 1 mL/min by capturing green fluorescent protein (GFP)-positive cells from blood. Further capturing of CTCs from metastatic cancers patients revealed a positive capture rate of 83.3%. Furthermore, our device was compared with CellSearch system via parallel analysis of 30 cancer patients, to find no significant difference between the capture efficiency of both methods. However, our device displayed advantage in terms of time, sample volume and cost for analysis. Thus, our integrated device with sterile environment and convenient use will be a promising platform for CTCs detection with potential clinical application.