Carp horizontal cells in culture respond selectively to L-glutamate and its agonists.
Carp horizontal cells in culture respond selectively to L-glutamate and its agonists.
复制标题
培养的鲤鱼水平细胞选择性地响应 L-谷氨酸及其激动剂。
DOI：
10.1073/pnas.79.3.936
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发表时间：
1982
影响因子：
11.1
通讯作者：
Dowling,JE
中科院分区：
文献类型：
--
作者：
Lasater,EM;Dowling,JE
从鲤鱼视网膜中酶解分离水平细胞，培养2-7天。培养的水平细胞通常静息膜电位为-50至-70 mV，输入电阻为100-150 m ω。用一些神经递质药物及其类似物处理这些细胞。只有3,4-二羟基苯丙氨酸（多巴胺）、l -谷氨酸和某些谷氨酸类似物才会引起显著的反应。对多巴胺的反应不一致；大多数情况下，膜超极化和输入电阻增加。然而，对l -谷氨酸及其类似物，准质酸盐和盐酸盐的高度特征性反应在几乎所有的细胞中都被观察到。反应包括最初的梯度去极化伴随着电阻增加，随后在大多数情况下延长（1至2分钟）再生去极化。反应的再生成分似乎是Ca2+依赖的，而潜在的梯度电位可能是由于膜的K+电导降低。
Horizontal cells were enzymatically isolated from the carp retina and maintained in culture for 2-7 days. Cultured horizontal cells typically had resting membrane potentials of -50 to -70 mV and input resistances of 100-150 m omega. The cells were treated with a number of neurotransmitter agents and their analogues. Significant responses were evoked only by 3,4-dihydroxyphenylalanine (dopamine), L-glutamate, and certain glutamate analogues. The responses to dopamine were inconsistent; most often, the membrane hyperpolarized and input resistances increased. However, highly characteristic responses to L-glutamate and its analogues, quisqualate and kainate, were observed in virtually all of the cells tested. The responses consisted of an initial graded depolarization accompanied by a resistance increase, followed in most cases by a prolonged (1- to 2-min) regenerative depolarization. The regenerative component of the response appears to be Ca2+ dependent, while the underlying graded potential may be due to a decrease in K+ conductance of the membrane.