Exosomes derived from interleukin-10-treated dendritic cells can inhibit trinitrobenzene sulfonic acid-induced rat colitis
Exosomes derived from interleukin-10-treated dendritic cells can inhibit trinitrobenzene sulfonic acid-induced rat colitis
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白细胞介素10处理的树突状细胞衍生的外泌体可以抑制三硝基苯磺酸诱导的大鼠结肠炎
DOI：
10.3109/00365521.2010.490596
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发表时间：
2010-09
影响因子：
1.9
通讯作者：
Jiang, Hong
中科院分区：
文献类型：
--
作者：
Meng, Song;Wu, Wenxi;Chen, Tao;Yang, Xiaojun;Jiang, Hong
抽象目标。炎症性肠病（Inflammatory bowel disease，IBD）是以胃肠道慢性炎症为特征的疾病，主要指克罗恩病和溃疡性结肠炎。最近的报道表明，来源于白细胞介素-10（IL-10）处理的骨髓来源的树突状细胞（DC）的外泌体可以降低小鼠中已建立的胶原诱导的关节炎（CIA）的发病率和严重程度。基于IL-10在正常粘膜免疫发展中的重要作用，我们研究了来源于用IL-10处理的DC的外泌体（称为IL-10-外泌体）是否可以抑制三硝基苯磺酸（TNBS）诱导的结肠炎。材料和方法。我们使用大鼠TNBS诱导的结肠炎模型来解决IL-10-外泌体在体内的治疗潜力。更具体地，向Wistar大鼠施用TNBS的直肠灌肠剂，并在第3天腹膜内注射IL-10-外泌体。结果在高水平的主要组织相容性复合物II类（MHC II）和低水平的共刺激分子和膜结合IL-10表达的背景下，IL-10-外泌体治疗显著降低了TNBS诱导的结肠炎的所有分析的临床、宏观和组织病理学参数。IL-10-exosomes的治疗作用与下调结肠组织中IL-2、IFN-γ和TNF-α的mRNA表达有关。重要的是，用IL-10-外泌体处理导致结肠组织中IL-10 mRNA表达和结肠固有层中调节性T细胞（TcB）的显著上调。结论.结果表明，IL-10-exosomes治疗可以抑制急性TNBS诱导的结肠炎，并可能为IBD提供一种有前途的新治疗策略。
Abstract Objective. Inflammatory bowel disease (IBD), which mainly refers to Crohn's disease and ulcerative colitis, is characterized by chronic inflammation of the gastrointestinal tract. Recent reports have demonstrated that exosomes derived from interleukin-10 (IL-10)-treated bone marrow-derived dendritic cells (DCs) can reduce the incidence and severity of established collagen-induced arthritis (CIA) in mice. Based on the essential role of IL-10 in the development of normal mucosal immunity, we investigated whether exosomes derived from DCs treated with IL-10 (known as IL-10-exosomes) can suppress the trinitrobenzene sulfonic acid (TNBS)-induced colitis. Material and Methods. We used the rat TNBS-induced colitis model to address the therapeutic potential of IL-10-exosomes in vivo. More specifically, a rectal enema of TNBS was administered to Wistar rats, and IL-10-exosomes were injected intraperitoneally on Day 3. Results. In the context of a high level of major histocompatibility complex class II (MHC II) and a low level of co-stimulatory molecule and membrane-bound IL-10 expression, IL-10-exosomes treatment substantially reduced all analyzed clinical, macroscopic, and histopathologic parameters of TNBS-induced colitis. The therapeutic effects of IL-10-exosomes were associated with a down-regulation mRNA expression of IL-2, IFN-γ and TNF-α in colon tissues. Importantly, treatment with IL-10-exosomes resulted in a pronounced up-regulation of IL-10mRNA expression in colon tissues and regulatory T cells (Tregs) in Colonic lamina propria. Conclusions. The results suggest that IL-10-exosomes treatment can suppress acute TNBS-induced colitis and may offer a promising new therapeutic strategy for IBD.
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影响因子：
4.4
作者：
Haiying Liu;Bin Hu;Damo Xu;F. Liew
通讯作者：
Haiying Liu;Bin Hu;Damo Xu;F. Liew
影响因子：
2.2
作者：
VANDERHEIJDEN, PJ;STOK, W
通讯作者：
STOK, W
DOI：
10.1016/s0016-5085(98)83795-5
发表时间：
1998-04
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
G. Hartmann;C. Bidlingmaier;B. Siegmund;S. Albrich;J. Schulze;K. Tschoep;A. Eigler;H. Lehr
通讯作者：
G. Hartmann;C. Bidlingmaier;B. Siegmund;S. Albrich;J. Schulze;K. Tschoep;A. Eigler;H. Lehr
影响因子：
14.8
作者：
Wirtz, Stefan;Neufert, Clemens;Neurath, Markus F.
通讯作者：
Neurath, Markus F.
DOI：
10.4049/jimmunol.169.5.2284
发表时间：
2002-09
期刊：
The Journal of Immunology
影响因子：
--
作者：
C. Voisine;F. Hubert;B. Trinité;M. Heslan;R. Josien
通讯作者：
C. Voisine;F. Hubert;B. Trinité;M. Heslan;R. Josien