ten-1, an essential gene for germ cell development, epidermal morphogenesis, gonad migration, and neuronal pathfinding in Caenorhabditis elegans
ten-1, an essential gene for germ cell development, epidermal morphogenesis, gonad migration, and neuronal pathfinding in Caenorhabditis elegans
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DOI：
10.1016/j.ydbio.2005.02.017
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发表时间：
2005-06-01
影响因子：
2.7
通讯作者：
Chiquet-Ehrismann, R
中科院分区：
文献类型：
--
作者：
Drabikowski, K;Trzebiatowska, A;Chiquet-Ehrismann, R
10 -m （odz）是在果蝇中发现的唯一一个编码跨膜蛋白而不是转录因子的成对规则基因。脊椎动物的Ten-m同源物与模式形成和神经元发育有关。为了研究该蛋白在发育中的作用，我们在这里描述了秀丽隐杆线虫同源物10 -1的结构和功能。我们发现两个启动子控制两个不同的10 -1转录本的表达。这导致II型跨膜蛋白变体在其细胞内结构域的表达不同。这两个10 -1转录本在发育和成年过程中表现出复杂但不同的表达模式。通过RNAi实验干扰Ten-1表达导致多种表型，导致皮下细胞迁移、神经元迁移、寻径和束化、远端尖端细胞迁移、体细胞性腺建立和配子体发生等缺陷。通过对缺失突变体的分析证实了RNAi表型，该突变体表明10 -1是性腺形成所必需的。在细胞膜和细胞核中检测到长形式的胞内结构域。我们认为10 -1作为形态发生线索的受体，在其蛋白水解从细胞膜释放后，通过将其胞内结构域易位到细胞核，直接向细胞核发出信号。(c) 2005爱思唯尔公司版权所有。
ten-m (odz) is the only pair-rule gene discovered in Drosophila that encodes a transmembrane protein and not a transcription factor. The vertebrate Ten-m orthologues have been implicated in pattern formation and neuronal development. To investigate the role of this protein in development, we characterize here the structure and function of the Caenorhabditis elegans orthologue ten-1. We found that two promoters control the expression of two different ten-1 transcripts. This results in the expression of type II transmembrane protein variants differing in their intracellular domains. Both ten-1 transcripts show complex, but distinct, expression patterns during development and in the adult. Interference with Ten-1 expression by RNAi experiments leads to multiple phenotypes resulting in defects in hypodermal cell migration, neuronal migration, pathfinding and fasciculation, distal tip cell migration, the establishment of the somatic gonad, and gametogenesis. The RNAi phenotypes were confirmed by the analysis of a deletion mutant which revealed that Ten-1 is essential for somatic gonad formation. The intracellular domain of the long form was detected at the cell membrane and in the nucleus. We propose that Ten-1 acts as a receptor for morphogenetic cue(s) and directly signals to the nucleus by translocation of its intracellular domain to the nucleus following its proteolytic release from the cell membrane. (c) 2005 Elsevier Inc. All rights reserved.