Facile and definitive determination of human adriamycin and daunoribicin metabolites by high-pressure liquid chromatography.
Facile and definitive determination of human adriamycin and daunoribicin metabolites by high-pressure liquid chromatography.
复制标题
通过高压液相色谱法轻松、准确地测定人阿霉素和柔红霉素代谢物。
DOI：
10.1124/dmd.8.3.152
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
N. Bachur
中科院分区：
文献类型：
--
作者：
P. Andrews;D. Brenner;F. Chou;H. Kubo;N. Bachur
在muBondapak-Phenyl色谱柱上，以甲酸铵/四氢呋喃为溶剂体系，对阿霉素及其代谢产物进行了分离和表征。用同样的方法分离并鉴定了柔红霉素和7种柔红霉素代谢产物。用氯仿/2-丙醇（1：1）从人血浆中以50-70%的效率提取阿霉素及其主要代谢产物阿霉素，并以柔红霉素内标物定量。同时测定了母体药物阿霉素和4-O-硫酸盐的尿浓度。从人血浆中提取柔红霉素和柔红霉素酚，并根据阿霉素内标物进行定量。荧光检测系统的检测限为1.0 pmol，血浆中可测量低至25 mmol/ml的水平。
Adriamycin and eight metabolites were separated and characterized with an ammonium formate/tetrahydrofuran solvent system on a muBondapak-Phenyl column. Danorubicin and seven daunorubicin metabolites were separated and characterized by the same method. Adriamycin and its major metabolite, adriamycinol, were extracted with 50-70% efficiency from human plasma with chloroform/2-propanol (1:1) and quantified against a daunorubicin internal standard. Urinary concentrations of parent drug, adriamycinol, and the 4-O-sulfate were determined as well. Daunorubicin and daunorubicinol were extracted from human plasma and quantified against an adriamycinol internal standard. The fluorescence detection system has a detection limit of 1.0 pmol, and levels as low as 25 mmol/ml are measurable in plasma.