Mouse strain differences in eosinophilic airway inflammation caused by intratracheal instillation of mite allergen and diesel exhaust particles
Mouse strain differences in eosinophilic airway inflammation caused by intratracheal instillation of mite allergen and diesel exhaust particles
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DOI：
10.1002/jat.949
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Shibamoto, T
中科院分区：
文献类型：
--
作者：
Ichinose, T;Takano, H;Shibamoto, T
研究了不同品系小鼠对粉尘螨和柴油机尾气颗粒物的反应差异。小鼠品系BALB/c、ICR和C3 H/He接受1 μ g D. farinae或1杯D.花粉+50微克的DEP，以2周为间隔，经鼻内给药4次。粉尘螨处理引起嗜酸性粒细胞和淋巴细胞的募集。嗜酸性粒细胞气道炎症的数量级为BALB/c < 1CR <C3 H/He小鼠。肺组织中eotaxin和IL-5蛋白水平与D.粉尘给药。柴油机尾气颗粒物通过气道杯状细胞增殖加重了嗜酸性粒细胞炎症的表现，并增强了三种小鼠中嗜酸性粒细胞趋化因子和IL-5的局部表达。肺组织中eotaxin和IL-5水平与D.花粉+ DEP. D. farinae或D.粉尘+ DEP组为BALB/c < 1CR <C3 H/He小鼠。DEP对C3 H/He小鼠IgG 1的产生有明显的佐剂效应（P < 0.05）。这些结果表明，小鼠菌株的差异，在生产嗜酸性气道炎症的D。farinae + DEP与IL-5和eotaxin的局部表达差异有关。DEP的增强作用可能是通过局部表达的细胞因子增加介导的。抗原特异性IgG 1可能是DEP增强过敏性哮喘发病机制的重要免疫球蛋白。版权所有（C）2004约翰威利父子有限公司。
Response differences by different strains of mice towards house dust mites (Dermatophagoides farinae) or diesel exhaust particles (DEP) were investigated. Mouse strains BALB/c, ICR and C3H/He received 1 mug of D. farinae or 1 mug of D. farinae + 50 mug of DEP intratracheally four times at 2-week intervals. Dermatophagoides farinae treatment caused the recruitment of eosinophils and lymphocytes. The order of magnitude of the eosinophilic airway inflammation was BALB/c < 1CR < C3H/He mice. The protein levels of eotaxin and IL-5 in lung tissues correlated with the manifestations of eosinophilic airway inflammation by D. farinae administration. Diesel exhaust particles aggravated the manifestation of the eosinophilic inflammation through goblet cell proliferation in the airway and enhanced the local expression of eotaxin and IL-5 in all three strains of mice. The levels of eotaxin and IL-5 in lung tissues corresponded to the pathological changes caused by D. farinae + DEP. The increasing order of production levels of antigen-specific IgG1 by D. farinae or D. farinae + DEP was BALB/c < 1CR < C3H/He mice. The significant adjuvant effect of DEP on IgG1 production was observed in the C3H/He mice (P < 0.05). These results suggest that the murine strain differences in the production of eosinophilic airway inflammation by D. farinae + DEP are related to differences in local expression of IL-5 and eotaxin. The enhancing effects of DEP may be mediated by a cytokine increase in the local expression. Antigen-specific IgG1 may be an important immunoglobulin in the pathogenesis of allergic asthma enhanced by DEP. Copyright (C) 2004 John Wiley & Sons, Ltd.