Herpesvirus cyprini: a search for viral genome in infected fish by infected fish by in situ hybridization
Herpesvirus cyprini: a search for viral genome in infected fish by infected fish by in situ hybridization
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鲤鱼疱疹病毒：通过原位杂交在受感染的鱼中寻找病毒基因组
DOI：
10.1111/j.1365-2761.1993.tb00883.x
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
T. Sano
中科院分区：
文献类型：
--
作者：
N. Sano;M. Moriwake;R. Hondo;T. Sano
.在鲤鱼中追踪了鲤疱疹病毒（CHV）基因组，用生物素标记的探针原位杂交法检测急性感染后的细胞凋亡。在包括脑神经节在内的几种组织中检测到病毒基因组。皮下组织和脊神经。然而，在此阶段，未检测到病毒抗原，也未分离出病毒。当存在含有病毒抗原甚至感染性病毒颗粒的乳头状瘤时，在相同的鱼组织中也检测到病毒基因组。在乳头状瘤消退后，在这些组织中仍然检测到病毒基因组。提示CHV潜伏于脑神经节、皮下组织和脊神经，并与乳头状瘤的诱发和复发有关。
. Herpesvirus cyprini (CHV) genome was traced in carp, Cyprinus carpio L., after acute infection by the method of in situ hybridization with biotinylated probes. The viral genome was detected in several tissues including cranial nerve ganglia. Subcutaneous tissue and spinal nerves. However, at this stage, viral antigens were not detected and the virus was not isolated. The viral genome was also detected in the same fish tissues when papillomas were present which contained viral antigens and even infective virus particles. After papilloma regression, the viral genome was still detected in these tissues. It is suggested that CHV becomes latently established in cranial nerve ganglia, subcutaneous tissue and spinal nerves, and is associated with the induction and recurrence of papillomas.