Long RNA hairpins that contain inosine are present in Caenorhabditis elegans poly(A)+ RNA
Long RNA hairpins that contain inosine are present in Caenorhabditis elegans poly(A)+ RNA
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DOI：
10.1073/pnas.96.11.6048
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发表时间：
1999-05-25
影响因子：
11.1
通讯作者：
Bass, BL
中科院分区：
文献类型：
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作者：
Morse, DP;Bass, BL
作用于 RNA 的腺苷脱氨酶 (ADAR) 是一种 RNA 编辑酶，可将双链 RNA 中的腺苷转化为肌苷。自 ADAR 发现以来的 12 年里，仅鉴定了少数天然底物。当基因组编码的腺苷被鉴定为 cDNA 中的鸟苷时，这些底物是偶然发现的。为了加深我们对 ADAR 生物学作用的理解，我们开发了一种系统识别 ADAR 底物的方法。在我们对该方法的首次应用中，我们在秀丽隐杆线虫中鉴定出了另外五种底物。其中四种底物是在非翻译区域编辑的 mRNA，一种是在整个长度上进行编辑的非编码 RNA。编辑后的区域预计会形成长发夹结构，其中一个 RNA 编码 POP-1，这是一种参与细胞命运决定的蛋白质。
Adenosine deaminases that act on RNA (ADARs) are RNA-editing enzymes that convert adenosine to inosine within double-stranded RNA. In the 12 years since the discovery of ADARs only a few natural substrates have been identified. These substrates were found by chance, when genomically encoded adenosines were identified as guanosines in cDNAs. To advance our understanding of the biological roles of ADARs, we developed a method for systematically identifying ADAR substrates. In our first application of the method, we identified five additional substrates in Caenorhabditis elegans. Four of those substrates are mRNAs edited in untranslated regions, and one is a noncoding RNA edited throughout its length. The edited regions are predicted to form long hairpin structures, and one of the RNAs encodes POP-1, a protein involved in cell fate decisions.