Re-thinking miRNA-mRNA interactions: intertwining issues confound target discovery.
Re-thinking miRNA-mRNA interactions: intertwining issues confound target discovery.
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DOI：
10.1002/bies.201400191
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发表时间：
2015-04
期刊：
影响因子：
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通讯作者：
Cloonan N
中科院分区：
文献类型：
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作者：
Cloonan N
尽管有一个图书馆充满了关于miRNA生物学的文献，但与miRNA靶点检测、生物学效应和作用模式相关的核心问题仍然存在争议。这篇文章提出，直接miRNA作用的主要机制是翻译抑制，而大部分miRNA作用是基于mRNA的。它探讨了几个问题混淆miRNA靶检测，并讨论了他们的影响，占主导地位的“miRNA种子”教条和探索非典型的结合位点。最后，它比较了miRNA靶预测和转录因子结合预测，并质疑了基于哪些miRNA核苷酸与mRNA配对来表征miRNA结合位点的价值。
Despite a library full of literature on miRNA biology, core issues relating to miRNA target detection, biological effect, and mode of action remain controversial. This essay proposes that the predominant mechanism of direct miRNA action is translational inhibition, whereas the bulk of miRNA effects are mRNA based. It explores several issues confounding miRNA target detection, and discusses their impact on the dominance of “miRNA seed” dogma and the exploration of non-canonical binding sites. Finally, it makes comparisons between miRNA target prediction and transcription factor binding prediction, and questions the value of characterizing miRNA binding sites based on which miRNA nucleotides are paired with an mRNA.