Macrophage colony stimulating factor controls macrophage recruitment to the cycling mouse uterus.
Macrophage colony stimulating factor controls macrophage recruitment to the cycling mouse uterus.
复制标题
巨噬细胞集落刺激因子控制巨噬细胞向循环小鼠子宫的募集。
DOI：
10.1016/0012-1606(92)90140-c
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发表时间：
1992
影响因子：
2.7
通讯作者：
Choudhuri,R
中科院分区：
文献类型：
--
作者：
Wood,GW;De,M;Sanford,T;Choudhuri,R
孕激素和雌激素诱导子宫上皮细胞产生巨噬细胞集落刺激因子（CSF-1），小鼠子宫上皮细胞在胚胎发育过程中产生大量CSF-1。目前的研究表明，雌激素和孕激素各自诱导子宫CSF-1基因的转录和翻译检测的北方印迹和生物测定。子宫内CSF-1的生产是更大的存在下，雌激素和孕激素比单独暴露于任何激素。CSF-1 mRNA在整个发情周期的子宫中检测，而CSF-1的生物活性仅在发情前期检测。CSF-1的产生与子宫中巨噬细胞的存在直接相关。卵巢切除术后，子宫CSF-1基因转录迅速丧失，子宫巨噬细胞数量也急剧减少。子宫CSF-1和子宫巨噬细胞通过全身给予雌激素或孕酮以剂量依赖性方式重建。高浓度的循环雌激素和孕激素增加了子宫内巨噬细胞的数量，并增加了它们在上皮表面附近的积累。在骑自行车的小鼠子宫中也观察到类似的关系。在向卵巢切除小鼠腔内注射重组人CSF-1后，巨噬细胞在子宫中积累，直接证明CSF-1从外周血募集巨噬细胞进入子宫的能力。这些研究表明，雌激素和孕激素刺激小鼠子宫上皮细胞产生CSF-1，控制动情周期中子宫内巨噬细胞的募集和分布。
Progesterone and estrogen induce uterine epithelial cells to produce macrophage colony stimulating factor (CSF-1), and mouse uterine epithelial cells produce large amounts of CSF-1 during embryo development. The present study demonstrated that estrogen and progesterone each induce uterine CSF-1 gene transcription and translation detectable by Northern blotting and bioassay. Intrauterine CSF-1 production was greater in the presence of both estrogen and progesterone than following exposure to either hormone alone. CSF-1 mRNA was detectable in the uterus throughout the estrous cycle while CSF-1 bioactivity was detected only during proestrus. CSF-1 production was directly related to the presence of macrophages in the uterus. Ovariectomy, which was rapidly followed by a loss of uterine CSF-1 gene transcription, also was followed by a dramatic decrease in the number of uterine macrophages. Both uterine CSF-1 and uterine macrophages were reconstituted in a dose-dependent manner by systemic administration of estrogen or progesterone. High concentrations of circulating estrogen and progesterone increased the number of macrophages in the uterus and increased their accumulation near epithelial surfaces. Similar relationships were observed in the uterus of cycling mice. Macrophages accumulated in the uterus following intraluminal injection of recombinant human CSF-1 to ovariectomized mice, directly demonstrating the ability of CSF-1 to recruit macrophages from peripheral blood into the uterus. These studies demonstrated that estrogen and progesterone stimulation of CSF-1 production by mouse uterine epithelial cells controls recruitment and distribution of macrophages in the uterus during the estrous cycle.