Oviposition deterrents in larval frass of the cotton boll worm, Helicoverpa armigera (Lepidoptera: Noctuidae): chemical identification and electroantennography analysis.
Oviposition deterrents in larval frass of the cotton boll worm, Helicoverpa armigera (Lepidoptera: Noctuidae): chemical identification and electroantennography analysis.
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DOI：
10.1016/j.jinsphys.2005.11.011
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发表时间：
2006-03
影响因子：
2.2
通讯作者：
Haiyan Xu;Guoqing Li;Mulan Liu;G. Xing
中科院分区：
文献类型：
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作者：
Haiyan Xu;Guoqing Li;Mulan Liu;G. Xing
采用行为生物测定和触角电位分析方法，研究了棉铃虫（CBW）、棉铃虫（Helicoverpa armigera）幼虫取食人工饲料（FA）和棉花叶片（FC）后的粪便中的产卵抑制物质。结果发现，水悬浮液或已烷提取物的粪便FA或FC，在相应的食物，显着阻止产卵的同种。当粪便FA和FC的己烷提取物进一步分配为极性和中性脂质部分时，两种极性部分显着减少产卵。来自粪便FC的中性级分也表现出显著的威慑，尽管活性远低于相应的极性级分。极性脂质组分含有几种脂肪酸，主要是比例接近1：1的棕榈酸和油酸。在粪便FA或FC中发现的相同组成的真实脂肪酸的混合物模仿了阻止效果。此外，这些脂肪酸和它们的混合物中发现的比例在粪便FA或FC引起显着的触角电位反应和典型的剂量-反应曲线。因此，这表明CBW幼虫可能部署两种类型的产卵抑制剂：一个非特异性和一个特定的。前者是脂肪酸的混合物，不依赖于食物，在产卵威慑中起重要作用，而后者可能只产生于幼虫取食棉花叶时。对这种部署的可能解释也进行了讨论。
Oviposition deterrents in the frass of cotton bollworm (CBW), Helicoverpa armigera larvae fed on an artificial diet (FA) and on cotton Gossypium hirsutum leaves (FC) were investigated by behavioral bioassays and electroantennography analyses in the laboratory. It was found that a water suspension or a hexane extract of the frass FA or FC, in contrast to the corresponding foods, significantly deterred oviposition of conspecifics. When hexane extracts of the frass FA and FC were further partitioned into polar and neutral lipid fractions, two polar fractions significantly reduced oviposition. The neutral fraction from frass FC also exhibited significant deterrence, although the activity was much lower than that of the corresponding polar fraction. The polar lipid fractions contained several fatty acids, mainly palmitic and oleic acid at the ratio nearly 1:1. A blend of authentic fatty acids of the same composition found in frass FA or FC mimicked the deterring effect. Moreover, these fatty acids and their blend at the ratio found in frass FA or FC elicited significant electroantennogram responses and typical dose–response curves. Thus, it is suggested that CBW larvae may deploy two types of oviposition deterrents: a non-specific and a specific one. The former is a blend of fatty acids, independent of food and plays an important role in oviposition deterrence, whereas the latter may be produced only when the larvae feed on cotton leaves. The possible explanations of this deployment have also been discussed.