Molecular Genetic Methods for Detection and Identification of Viable but Nonculturable Microorganisms

Molecular Genetic Methods for Detection and Identification of Viable but Nonculturable Microorganisms
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用于检测和鉴定活的但不可培养的微生物的分子遗传学方法

DOI:
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发表时间:
2000
期刊:
影响因子:
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通讯作者:
I. Knight
I. Knight
中科院分区:
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作者:
I. Knight

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与用于检测和鉴定临床和环境样品中的微生物的基于培养的方法相关的问题促使开发不需要培养靶生物的替代方法。特异性靶向微生物核酸的分子遗传学方法已成为鉴定培养和未培养微生物的重要工具。这些方法的灵敏度接近基于培养的方法的灵敏度,并且这些方法的特异性通常超过基于培养的方法是可以理解的。事实上,分子遗传学方法已经成为一套强大的工具,许多研究人员使用它来检测和鉴定可培养的微生物,在他们的生活,但noncultureable状态,以及生物体尚未被带入文化。
The problems associated with culture-based methods for detection and identification of microorganisms in clinical and environmental samples have motivated the development of alternative methods which do not require cultivation of the target organisms. Molecular genetic methods, which specifically target microbial nucleic acids, have become important tools for the identification of both cultured and uncultured microorganisms. The sensitivity of these methods approaches that of culture-based methods and it is arguable that the specificity of these methods generally exceeds that of culture-based methods. Indeed, molecular genetic methods have become a powerful set of tools used by many investigators to detect and identify culturable microorganisms in their viable but nonculturable state, as well as organisms which have yet to be brought into culture.
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影响因子: --
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