Efficient identification and analysis of chemical exchange in biomolecules by R1rho relaxation dispersion with Amaterasu
Efficient identification and analysis of chemical exchange in biomolecules by R1rho relaxation dispersion with Amaterasu
复制标题
利用 Amaterasu 的 R1rho 弛豫分散体有效识别和分析生物分子中的化学交换
DOI：
10.1093/bioinformatics/btw188
复制
发表时间：
2016
期刊：
影响因子：
5.8
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Walinda;E. Morimoto;D. Nishizawa;M. Shirakawa;M. Sugase;K.
摘要：本文介绍了一种新的基于交叉极化的一维旋转标架弛豫色散核磁共振实验的采集和处理方法。这一易于使用的协议极大地方便了对大的开和离共振核磁共振数据集的筛选、获取、处理和模型拟合，以自动方式分析生物分子中的化学交换现象。可用性和实施：www.moleng.kyoto-u.ac.jp/∼moleng_01/amaterasu.Contact：ρ@moleng.kyoto-u.ac.jp上提供了包括电子表格、布鲁克脉冲程序和分析软件在内的Amatasupack
Summary:We introduce here a novel acquisition and processing methodology for cross-polarization based 1D rotating-frame relaxation dispersion NMR experiments. This easy-to-use protocol greatly facilitates the screening, acquisition, processing and model fitting of large on- and off-resonanceR1ρrelaxation dispersion NMR datasets in an automated manner for the analysis of chemical exchange phenomena in biomolecules.Availability and Implementation:TheAmaterasupackage including the spreadsheet, Bruker pulse programs and analysis software is available at www.moleng.kyoto-u.ac.jp/∼moleng_01/amaterasu.Contact: sugase@moleng.kyoto-u.ac.jp