The embryonic ontogeny of the gonadal somatic cells in mice and monkeys
The embryonic ontogeny of the gonadal somatic cells in mice and monkeys
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DOI：
10.1016/j.celrep.2021.109075
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发表时间：
2021-05-04
期刊：
影响因子：
8.8
通讯作者：
Saitou, Mitinori
中科院分区：
文献类型：
--
作者：
Sasaki, Kotaro;Oguchi, Akiko;Saitou, Mitinori
在胎儿早期，性腺是双能的，直到后来才变成卵巢或睾丸，具体取决于遗传性别。尽管许多研究探讨了生物电位性腺如何进行性别决定，但人们对双电位性腺及其祖细胞的空间和时间组织知之甚少。在这里，通过对小鼠进行谱系追踪，我们发现性腺起源于 T+ 原条，通过 WT1(+) 后中间中胚层，并且似乎在前面与肾上腺共享起源，在后面与后肾间质共享起源。小鼠和食蟹猴胚胎的比较单细胞转录组分析揭示了谱系轨迹和遗传程序的趋同，伴随着生物潜能性腺祖细胞的规范。该过程涉及上皮基因的持续表达和间质基因的上调，从而赋予上皮-间质混合状态。我们的研究为了解小鼠和灵长类动物的早期性腺发生提供了关键资源。
In the early fetal stage, the gonads are bipotent and only later become the ovary or testis, depending on the genetic sex. Despite many studies examining how sex determination occurs from biopotential gonads, the spatial and temporal organization of bipotential gonads and their progenitors is poorly understood. Here, using lineage tracing in mice, we find that the gonads originate from T+ primitive streak through WT1(+) posterior intermediate mesoderm and appear to share origins anteriorly with the adrenal glands and posteriorly with the metanephric mesenchyme. Comparative single-cell transcriptomic analyses in mouse and cynomolgus monkey embryos reveal the convergence of the lineage trajectory and genetic programs accompanying the specification of biopotential gonadal progenitor cells. This process involves sustained expression of epithelial genes and upregulation of mesenchymal genes, thereby conferring an epithelial-mesenchymal hybrid state. Our study provides key resources for understanding early gonadogenesis in mice and primates.