ISOLATION AND CHARACTERIZATION OF METASTATIC VARIANTS FROM HUMAN TRANSITIONAL-CELL CARCINOMA PASSAGED BY ORTHOTOPIC IMPLANTATION IN ATHYMIC NUDE-MICE
ISOLATION AND CHARACTERIZATION OF METASTATIC VARIANTS FROM HUMAN TRANSITIONAL-CELL CARCINOMA PASSAGED BY ORTHOTOPIC IMPLANTATION IN ATHYMIC NUDE-MICE
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DOI：
10.1016/s0022-5347(01)66923-4
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发表时间：
1995-10-01
影响因子：
6.6
通讯作者：
KILLION, JJ
中科院分区：
文献类型：
--
作者：
DINNEY, CPN;FISHBECK, R;KILLION, JJ
目的：这些研究旨在建立一种人类（膀胱癌）原位模型。
材料与方法：将人膀胱癌细胞系253J植入无胸腺裸鼠的膀胱肌层。通过体内循环，我们筛选出2个变异细胞系：253J B - V，是在膀胱中连续传代5次后分离出的膀胱细胞系；253J lung - IV，是从一个经膀胱循环的肺肿瘤结节建立的。
结果：与亲代细胞系相比，这2个细胞系显示出更强的致瘤性，潜伏期缩短且生长迅速。此外，将这些细胞系原位植入导致了肺转移。这些体内筛选出的转移性细胞系表现出独特的核型改变、增强的非贴壁依赖性生长、碱性成纤维细胞生长因子的过度表达、黏附分子表达的改变以及穿过基质胶迁移的能力。
结论：这种可重复的人膀胱癌模型为研究与肿瘤进展和转移相关的细胞特性提供了机会，并且适用于评估浸润性膀胱癌的新治疗策略。
Purpose: These studies were designed to develop an orthotopic model for humanMaterials and Methods: The human bladder cancer cell line 253J was implanted into the muscular wall of the bladder of athymic nude mice. By in vivo recycling, we selected for 2 variant cell lines: 253J B-V, a bladder Line isolated after 5 serial passages in the bladder, and 253J lung-IV, established from a lung tumor nodule that was recycled through the bladder.Results: These 2 cell Lines showed enhanced tumorigenicity, as measured by a decreased latent period, and rapid growth as compared with the parental cell line. Moreover, orthotopic implantation of these cell lines resulted in metastasis to the lungs. These in vivo-selected, metastatic cell lines exhibited unique karyotypic alterations, increased anchorage-independent growth, overexpression of basic fibroblast growth factor, altered expression of adhesion molecules and the ability to migrate through Matrigel.Conclusions: This reproducible model of human bladder cancer offers the opportunity to study cellular properties associated with tumor progression and metastasis and is suitable for the evaluation of new therapeutic strategies for invasive bladder cancer.