Comparison of HPLC with electrochemical detection and LC-MS/MS for the separation and validation of artesunate and dihydroartemisinin in animal and human plasma
Comparison of HPLC with electrochemical detection and LC-MS/MS for the separation and validation of artesunate and dihydroartemisinin in animal and human plasma
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DOI：
10.1016/j.jchromb.2008.04.019
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发表时间：
2008-05-15
影响因子：
3
通讯作者：
Weina, Peter
中科院分区：
文献类型：
--
作者：
Gu, Yuanchao;Li, Qigui;Weina, Peter
高效液相色谱还原电化学检测（HPLC-ECD）方法自1985年开始用于青蒿素的测定。虽然该方法已得到显着改进，但具有显着优势的串联质谱（LC-MS/MS）系统已逐渐取代HPLC-ECD来分析血浆中的青蒿琥酯和双氢青蒿素。在本研究中，评估了两种方法的线性、定量限、选择性、精密度和准确度。 HPLC-ECD 在各种验证参数方面表现良好，并且在血浆中校准时与 LC-MS/MS 表现出良好的一致性。然而，LC-MS/MS 的主要优点是它只需要 HPLC-ECD 检测所需血浆体积的十分之一。由 Elsevier B.V. 出版
High-performance liquid chromatography with reductive electrochemical detection (HPLC-ECD) method has been used for assaying artemisinins since 1985. Although the methods have been remarkably improved, tandem mass spectrometry (LC-MS/MS) systems with significant advantages have gradually replaced HPLC-ECD to analyze artesunate and dihydroartemisinin in plasma. In the present study, the two methods were evaluated for linearity, quantitation limits, selectivity, precision, and accuracy. The HPLC-ECD performed well in terms of various validation parameters, and showed a good agreement with the LC-MS/MS when calibrated in plasma. However, the major benefit of LC-MS/MS is that it requires only one-tenth the plasma volume needed by HPLC-ECD assay. Published by Elsevier B.V.