Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells
Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells
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DOI：
10.1016/j.jcyt.2013.11.004
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发表时间：
2014-02-01
期刊：
影响因子：
4.5
通讯作者：
Wagner, Wolfgang
中科院分区：
文献类型：
--
作者：
Hemeda, Hatim;Giebel, Bernd;Wagner, Wolfgang
用于治疗性细胞增殖（例如间充质基质细胞（MSC））的培养基通常包含血清添加剂。传统上，在基础研究和大多数临床试验中补充胎牛血清。在过去的几年中，许多实验室将其培养条件适应于人血小板裂解物（hPL），这进一步刺激了MSC的增殖和扩增。特别是在临床应用方面，胎牛血清的人类替代品显然是优选的。hPL通过破坏血小板膜从人血小板单位产生，这通常通过重复冻融循环进行。众所周知，此类培养补充剂的定义不明确，许多参数都会导致hPL的批次间差异，例如不同量的血浆、广泛的生长因子和供体特异性效应。hPL的血浆成分需要加入抗凝血剂如肝炎，以防止hPL培养基的凝胶化，并且其浓度必须标准化。描述hPL的标签-如“无异种”、“无动物”和“无血清”-在文献中使用不一致，如果不严格评估，可能会产生误导。对相关生长因子、附着因子、microRNA和外来体的精确组成的进一步分析将为优化和确定的培养条件铺平道路。使用hPL有几个优点和缺点：必须考虑到它们，因为细胞培养添加剂的选择对细胞制备有重大影响。
Culture media for therapeutic cell preparations-such as mesenchymal stromal cells (MSCs)-usually comprise serum additives. Traditionally, fetal bovine serum is supplemented in basic research and in most clinical trials. Within the past years, many laboratories adapted their culture conditions to human platelet lysate (hPL), which further stimulates proliferation and expansion of MSCs. Particularly with regard to clinical application, human alternatives for fetal bovine serum are clearly to be preferred. hPL is generated from human platelet units by disruption of the platelet membrane, which is commonly performed by repeated freeze and thaw cycles. Such culture supplements are notoriously ill-defined, and many parameters contribute to batch-to-batch variation in hPL such as different amounts of plasma, a broad range of growth factors and donor-specific effects. The plasma components of hPL necessitate addition of anticoagulants such as hepatitis to prevent gelatinization of hPL medium, and their concentration must be standardized. Labels for description of hPL-such as "xenogen-free," "animal-free" and "serum free"-are not used consistently in the literature and may be misleading if not critically assessed. Further analysis of the precise composition of relevant growth factors, attachment factors, microRNAs and exosomes will pave the way for optimized and defined culture conditions. The use of hPL has several advantages and disadvantages: they must be taken into account because the choice of cell culture additive has major impact on cell preparations.