Efficient and Response Surface Optimized Aqueous Enzymatic Extraction of Camellia oleifera (Tea Seed) Oil Facilitated by Concurrent Calcium Chloride Addition
Efficient and Response Surface Optimized Aqueous Enzymatic Extraction of Camellia oleifera (Tea Seed) Oil Facilitated by Concurrent Calcium Chloride Addition
复制标题
通过同时添加氯化钙促进油茶（茶籽）油的高效和响应表面优化水酶法提取
DOI：
10.1002/aocs.12009
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发表时间：
2018-01-01
影响因子：
2
通讯作者：
Jiang, Lianzhou
中科院分区：
文献类型：
--
作者：
Meng, Xianghe;Ge, Hangli;Jiang, Lianzhou
采用响应面分析法研究了水酶法提取油茶籽油的工艺条件。当使用0.80%纤维素酶（v/w）溶液（pH 6.0）在50 ℃下保持1小时，然后使用0.70% Alcalase（R）溶液（pH 9.2）在57 ℃下保持4.1小时进行2步AEE方法时，获得类似于93.5%的最大油回收率。结果发现，在蛋白质水解阶段添加Ca 2+使游离油产率从接近62.1%提高到接近86.6%。这是由于茶皂素的去除、阴离子多糖的交联以及钙离子对乳状液的破坏。这通过降低乳膏乳剂和本体溶液中的茶皂苷和多糖水平以及降低乳剂级分来验证。经测定，在抑制乳液形成方面，以连续流向蛋白水解产物中添加CaCl 2溶液上级一次性或分批添加。CaCl 2的添加可以提供替代另外需要的更费力、耗时的破乳过程的手段。
This paper reports an efficient aqueous enzymatic extraction (AEE) method for Camellia oleifera seed oil with the aid of response surface analysis. A maximum oil recovery of similar to 93.5% was obtained when a 2-step AEE process was performed using 0.80% cellulase (v/w) solution at pH 6.0 maintained at 50 degrees C for 1 h followed by a solution of 0.70% Alcalase (R) with pH 9.2 at 57 degrees C for 4.1 h. It was found that the addition of Ca2+ during the proteolysis stage improved the free oil yield from similar to 62.1 to similar to 86.6%. This was attributed to the removal of tea saponins, cross-linkage of anionic polysaccharides, and destabilization of cream emulsion by Ca2+. This was verified by decreased tea saponin and polysaccharide levels in the cream emulsion and bulk solution as well as lowering of the emulsion fraction. It was determined that addition of CaCl2 solution in continuous flow to the proteolysate is superior to one-time or batch addition in inhibiting emulsion formation. The addition of CaCl2 may provide a means of replacing the more laborious, time-consuming demulsification process otherwise required.