Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans
Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans
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DOI：
10.1128/jcm.37.3.497-503.1999
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发表时间：
1999-03-01
影响因子：
9.4
通讯作者：
Gyles, CL
中科院分区：
文献类型：
--
作者：
Boerlin, P;McEwen, SA;Gyles, CL
研究了产滋贺毒素大肠杆菌的已知或推定毒力因子与人类疾病之间的关系。对来自118种血清型的237株分离株进行单变量分析和多变量逻辑回归分析，结果显示，存在紧密连接蛋白（eae）和滋贺毒素2（stx（2））基因与来自人类血清型的分离株之间存在显著相关性。在出血性结肠炎和溶血性尿毒症综合征中报告的血清型分离株中也发现了类似的相关性。肠出血性E.单因素分析显示，EHEC溶血素基因与重症患者血清型分离株相关，但多因素Logistic回归分析显示，EHEC溶血素基因与重症患者血清型分离株无关。紧密连接蛋白和肠出血性大肠杆菌溶血素基因之间的强关联可以解释在这些多变量模型中肠出血性大肠杆菌溶血素缺乏统计学显著性，但不能排除溶血素在人类或疾病中真正缺乏生物学意义。这一结果值得进一步研究这一主题。多变量分析揭示了eae和stx（2）基因之间的相互作用，从而支持粘附素intimin和滋贺毒素2之间协同作用的假设。从8个主要血清型的112个人类分离株中观察到stx（2）基因与疾病严重程度之间存在很强的统计学关联。对来自牛的77株分离株和属于6种主要血清型的91株人分离株进行比较，结果显示滋贺毒素1和EspP蛋白酶基因与牛分离株显著相关，人分离株（特别是来自腹泻患者和健康人）对HEp-2细胞培养物的粘附性增加。
Associations between known or putative virulence factors of Shiga toxin-producing Escherichia coli and disease in humans were investigated. Univariate analysis and multivariate logistic regression analysis of a set of 237 isolates from 118 serotypes showed significant associations between the presence of genes for intimin (eae) and Shiga toxin 2 (stx(2)) and isolates from serotypes reported in humans. Similar associations were found with isolates from serotypes reported in hemorrhagic colitis and hemolytic-uremic syndrome. The enterohemorrhagic E. coli (EHEC) hemolysin gene was significantly associated with isolates from serotypes found in severe diseases in univariate analysis but not in multivariate logistic regression models. A strong association between the intimin and EHEC-hemolysin genes may explain the lack of statistical significance of EHEC hemolysin in these multivariate models, but a true lack of biological significance of the hemolysin in humans or in disease cannot be excluded. This result warrants further investigations of this topic. Multivariate analysis revealed an interaction between the eae and stx(2) genes, thus supporting the hypothesis of the synergism between the adhesin intimin and Shiga toxin 2. A strong statistical association was observed between the stx(2) gene and severity of disease for a set of 112 human isolates from eight major serotypes. A comparison of 77 isolates of bovine origin and 91 human isolates belonging to six major serotypes showed significant associations of the genes for Shiga toxin 1 and EspP protease with bovine isolates and an increased adherence on HEp-2 cell cultures for human isolates, particularly from diarrheic patients and healthy persons.