Molecular changes in Trypanosoma evansi after treatment against trypanosomosis.
Molecular changes in Trypanosoma evansi after treatment against trypanosomosis.
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伊氏锥虫病治疗后的分子变化。
DOI：
10.17420/ap6602.251
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发表时间：
2020
影响因子：
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通讯作者：
S. Barghash
中科院分区：
文献类型：
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作者：
S. Barghash
治疗后的生理和病理变化都不能解释为什么在同一组实验治疗的动物中锥虫的毒力相关。此外，尽管注射了相同的伊文氏t细胞菌株，但它们的行为彼此相似，但与其他组不同。目前的研究旨在讨论伊瓦西锥虫分离株在治疗后是否会发生分子变化。本研究除采用治疗前注射的伊文氏体原始菌株外，还采用了10株经前期处理后保存的伊文氏体分离株。将这些分离株以等剂量腹腔接种于11组雄性白化大鼠。基于PCR-TR3/TR4特异性引物，讨论了实验结果及相关分子变化。本研究还完成了基因库中伊文氏布氏、布氏布氏、布氏布氏布氏和冈比亚布氏布氏布氏的提交菌株和参考菌株的比对、基因序列和系统发育分析。目前的结果从分子上评估了人血浆O+和A+对伊氏t虫的有效性和高度抗锥虫活性，以及它们的菌株如何从其原始序列转移到最接近的布氏t虫形式。与此同时，在其他植物提取物组中，伊凡斯弧菌像癌细胞一样逐渐增殖，并且变得更毒力，更接近伊凡斯弧菌的参考菌株。我们的数据进一步表明，治疗后的伊文氏绦虫是一种过敏性组。这也证实了抗锥虫体活性的特异性和分子变化的发生与治疗类型有关。
Neither physiological nor pathological changes following treatments explained why trypanosomes in the same group of experimentally treated animals correlated in virulence. Also, they behaved like each other but not similar to other groups despite the same T. evansi injected strain. The current study aims to discuss whether molecular changes might occur to Trypanosoma evansi isolates followed treatments are responsible for that difference or not. Ten preserved isolates from T. evansi after previous treatments besides the original strain of T. evansi that injected before treatments were used in the present study. These isolates were intraperitoneally inoculated in 11 groups of male Wister Albino rats with equal doses. Parasitological findings and the molecular changes accompanied were discussed along with the experiment based on PCR-TR3/TR4 specific-primers. The study also achieved alignments, gene sequence and phylogenetic analysis for submitted and reference strains belong to T. evansi, T. brucei, T. b. brucei, and T. b. gambiense deposited in GenBank. The present results assessed molecularly the effectiveness and highly antitrypanosomal activity of human plasmas O+ and A+ on T. evansi than others, and how their strains drifted from its original sequence to the nearest form of T. brucei. At the same time, T. evansi in other plant extract groups multiplied progressively like cancer cells and became more virulent and close to reference strains of T. evansi. Our data further indicated that T. evansi after treatment was a paraphyletic group. It also corroborated the antitrypanosomal activityspecificity and the molecular changes occurring were correlated to the type of treatment.