Peanut agglutinin high phenotype of activated CD8+ T cells results from de novo synthesis of CD45 glycans
Peanut agglutinin high phenotype of activated CD8+ T cells results from de novo synthesis of CD45 glycans
复制标题
DOI：
10.1074/jbc.m405629200
复制
发表时间：
2004-08-27
影响因子：
4.8
通讯作者：
Paulson, JC
中科院分区：
文献类型：
--
作者：
Amado, M;Yan, Q;Paulson, JC
在外周激活后，小鼠CD8(+) T细胞表现出花生凝集素（PNA）结合增加的特征，反映了细胞表面低羟化的o -连接聚糖（Galbeta1-3GalNAcalpha-O-Thr/Ser）表达增加。在本报告中，我们发现在活化的CD8(+) T细胞上表达的大多数PNA受体是由CD45携带的。其他糖蛋白（如CD8）和糖脂asialo-GM1也携带PNA受体，尽管程度要小得多。对参与唾液酰化/去唾液酰化途径的酶的分析表明，在活化的CD8(+) T细胞中，PNA受体的产生不是由于内源性唾液酰化酶的上调。相反，我们的研究结果表明，PNA（高）表型是由CD45重新合成的结果，CD45携带了唾液化的核心1 - o -聚糖。
Following activation in the periphery, murine CD8(+) T cells exhibit a characteristic increased binding of peanut agglutinin (PNA), reflecting an increased expression of hyposialylated O-linked glycans (Galbeta1-3GalNAcalpha-O-Thr/Ser) on the cell surface. In this report, we show that the majority of the PNA receptors expressed on activated CD8(+) T cells are carried by CD45. Other glycoproteins (e.g. CD8) and the glycolipid asialo-GM1 also carry PNA receptors, although to a much lesser extent. Analysis of enzymes involved in the sialylation/de-sialylation pathways showed that generation of PNA receptors in activated CD8(+) T cells is not due to up-regulation of endogenous sialidases. Instead, our results indicate that the PNA(high) phenotype results from de novo synthesis of CD45 carrying reduced sialylated core 1 O-glycans.