Comment on "Discrimination of shifts in a soil microbial community associated with TNT-contamination using a functional ANOVA of 16S rRNA hybridized to oligonucleotide microarrays".
Comment on "Discrimination of shifts in a soil microbial community associated with TNT-contamination using a functional ANOVA of 16S rRNA hybridized to oligonucleotide microarrays".
复制标题
评论“使用与寡核苷酸微阵列杂交的 16S rRNA 功能方差分析来区分与 TNT 污染相关的土壤微生物群落的变化”。
DOI：
10.1021/es062479m
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发表时间：
2007
影响因子：
11.4
通讯作者：
Noble,PeterA
中科院分区：
文献类型：
--
作者：
Pozhitkov,AlexE;Noble,PeterA
Eyers等人(1)非平衡热解离（NTD）曲线可以用来区分土壤微生物群落的变化。我们自己使用凝胶垫阵列技术的经验（2和参考文献）提出了另一种解释。在这封信中，我们考虑了他们使用的图像采集和数据处理算法，并根据他们的结果，质疑NTD方法与功能方差分析（FANOVA）是否能够检测社区的变化。在做出这样的声明之前，需要对一系列定义明确的混合物进行测试。Eyers等人使用的图像采集和滤波算法。(1)在先前的论文（2）中进行了评估，该论文表明网格放置显著影响初始信号强度（SI）和曲线形状（包括Td）。在图S-1和表S-1（支持性信息）中，我们发现只有15.6%的网格框架准确地放置在衬垫的中心。
Eyers et al.(1) suggested that nonequilibrium thermal dissociation (NTD) curves could be used to discriminate shifts of soil microbial communities. Our own experience with gel pad array technology (2 and refs within) suggests an alternative interpretation. In this correspondence, we consider the image acquisition and data processing algorithm they used, and, based on their results, question whether the NTD approach in conjunction with functional ANOVA (FANOVA) is capable of detecting community shifts. Testing the technology against a series of well-defined mixtures is needed before such claims can be made. The image acquisition and filtering algorithm used by Eyers et al.(1) was evaluated in a previous paper (2), which showed that the grid placement significantly influenced initial signal intensities (SIs) and shapes of the curves (including Td). In Figure S-1 and Table S-1 (Supporting Information), we show that only 15.6% of the grid frames are exactly placed in the center the pad.